College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Molecules. 2012 Jan 18;17(1):951-70. doi: 10.3390/molecules17010951.
Vinegar and wine processing of medicinal plants are two traditional pharmaceutical techniques which have been used for thousands of years in China. Tetrahydropalmatine (THP), dehydrocorydaline (DHC) and protopine are three major bioactive molecules in Rhizoma Corydalis. In this study, a simple and reliable HPLC method was developed for simultaneous analysis of THP, DHC and protopine in rat tissues after gastric gavage administration of Rhizoma Corydalis. The validated HPLC method was successfully applied to investigate the effect of wine and vinegar processing on the compounds' distribution in rat tissues. Our results showed that processing mainly affect the T(max) and mean residence time (MRT) of the molecules without changing their C(max) and AUC(0-24)( )(h) Vinegar processing significantly increased the T(max) of DHC in heart, kidney, cerebrum, cerebrellum, brain stem and striatum and prolonged the T(max) of protopine in brain. No significant changes were observed on the T(max) of THP in rat tissues after vinegar processing. Wine processing reduced the T(max) of protopine and DHC in liver and spleen and T(max) of protopine in lung, but increased the T(max) of THP in all the rat tissues examined. To our knowledge, this is the first report on the effects of processing on the tissue distribution of the bioactive molecules from Rhizoma Corydalis.
醋制和酒制是中国传统的两种炮制方法，几千年来一直被用于药用植物加工。延胡索中的原小檗碱（THP）、脱氢紫堇碱（DHC）和巴马汀是三种主要的生物活性分子。本研究建立了一种同时测定大鼠胃灌胃给予延胡索后组织中 THP、DHC 和巴马汀的简单可靠的 HPLC 方法。验证后的 HPLC 方法成功应用于考察醋制和酒制对化合物在大鼠组织中分布的影响。结果表明，炮制主要影响分子的 Tmax 和平均驻留时间（MRT），而不改变 Cmax 和 AUC(0-24)( )(h)。醋制显著增加了 DHC 在心脏、肾脏、大脑、小脑、脑干和纹状体中的 Tmax，并延长了脑中巴马汀的 Tmax。醋制后，THP 在大鼠组织中的 Tmax 没有明显变化。酒制降低了 DHC 和巴马汀在肝、脾中的 Tmax，以及巴马汀在肺中的 Tmax，但增加了所有检测的大鼠组织中 THP 的 Tmax。据我们所知，这是首次报道炮制对延胡索生物活性分子组织分布的影响。
