Chamel Gabriel, Abadie Jérôme, Albaric Olivier, Labrut Sophie, Ponce Frédérique, Ibisch Catherine
1 Department of Internal Medicine (Oncology), Veterinary Campus of VetAgro Sup, University of Lyon, Marcy L'Etoile, France.
2 LUNAM University, Oniris, Nantes-Atlantic College of Veterinary Medicine and Food Sciences and Engineering, UP AMaROC, Atlanpole-La Chantrerie, Nantes, France.
J Feline Med Surg. 2017 Apr;19(4):351-357. doi: 10.1177/1098612X15625355. Epub 2016 Jan 1.
Objectives The aim of the study was to describe the clinical outcome of 30 cats with non-ocular melanomas and to evaluate the association between clinical or pathological parameters and overall survival time. Methods The database of the animal histopathological laboratory of the National Veterinary School of Nantes (Oniris, Nantes, France) was retrospectively searched to identify cases of feline non-ocular melanomas between December 2009 and April 2014. For each case, clinical data, including signalment, location of the primary tumour, staging, treatment and outcome, were collected from the medical records or via interviews with referring veterinarians. Histological and immunohistochemical evaluation included mitotic index, cytonuclear atypias, junctional activity, Melan A and S100 immunostaining, and surgical margins. Univariate analysis to test the prognostic value of the different variables was performed by the Kaplan-Meier product limit method using the log-rank test of significance. Results Thirty cats were included in the study. Eleven had a cutaneous non-auricular melanoma, six had a tumour located on the pinna and 13 had a tumour in the oral cavity. Cats with auricular melanomas were significantly younger than cats with tumours in other locations. Location and presence of clinical signs were not of prognostic significance, but the achromic phenotype was significantly associated with a poorer prognosis. Twenty cats were treated with surgery and survived significantly longer than cats that received only medical treatment or that did not receive any treatment. According to our data, mitotic index, cytonuclear atypias, junctional activity, Melan A or S100 expression, and surgical margins were not associated with survival. Conclusions and relevance We show for the first time, in a large series, that the auricular form of melanoma affected significantly younger cats than other extraocular forms. Most feline non-ocular melanomas are malignant and achromic tumours are associated with a poorer prognosis. According to this study, surgery should be considered as a priority.
目的 本研究旨在描述30只患有非眼黑色素瘤的猫的临床结局，并评估临床或病理参数与总生存时间之间的关联。方法 对法国南特国立兽医学院（Oniris，南特）动物组织病理学实验室的数据库进行回顾性检索，以确定2009年12月至2014年4月间猫非眼黑色素瘤的病例。对于每例病例，从病历或通过与转诊兽医访谈收集临床数据，包括特征、原发性肿瘤位置、分期、治疗和结局。组织学和免疫组化评估包括有丝分裂指数、细胞核异型性、交界活性、Melan A和S100免疫染色以及手术切缘。采用Kaplan-Meier乘积限界法和对数秩检验显著性进行单变量分析，以检验不同变量的预后价值。结果 30只猫纳入本研究。11只患有皮肤非耳廓黑色素瘤，6只肿瘤位于耳廓，13只肿瘤位于口腔。患有耳廓黑色素瘤的猫比其他部位肿瘤的猫明显更年轻。肿瘤位置和临床症状的存在无预后意义，但无色表型与较差的预后显著相关。20只猫接受了手术治疗，其存活时间明显长于仅接受药物治疗或未接受任何治疗的猫。根据我们的数据，有丝分裂指数、细胞核异型性、交界活性、Melan A或S100表达以及手术切缘与生存无关。结论及意义 我们首次在一个大样本系列中表明，耳廓型黑色素瘤比其他眼外型黑色素瘤影响的猫明显更年轻。大多数猫非眼黑色素瘤是恶性的，无色肿瘤与较差的预后相关。根据本研究，应优先考虑手术治疗。
