College of Life Science, Capital Normal University, Beijing, 100048, People's Republic of China.
Key Laboratory of Bamboo and Rattan Science and Technology of the SFA, International Centre for Bamboo and Rattan, Beijing, 100102, People's Republic of China.
Appl Microbiol Biotechnol. 2016 Jun;100(11):4875-83. doi: 10.1007/s00253-015-7222-9. Epub 2016 Jan 15.
Hypocrellin A (HA), well known as one of the best natural pigments and bioactive agent to treat skin diseases, is further anticipated to play a vital role in photodynamic therapy (PDT) in anticancer and antiviral treatments. In this study, an HA-producing strain ZZZ816 (Shiraia sp.) was isolated from the moso bamboo (Phyllostachys edulis) seeds, and gamma irradiation was used to mutagenize spores of the original strain. After treatment with cobalt-60 gamma ((60)Coγ) with different doses (20, 50, 80, 100, 150, 180, 300, and 500 Gy), the 100 Gy was selected as the optimal condition, which led to 77.2 % lethality of spores and 35 % positive mutant frequency. The extracted compound of the most excellent HA-producing strain (H-4-2) was precisely analyzed by a combination of seven detection methods, and the maximum HA content was shown to reach 2018.3 mg/L. HA production in H-4-2 increased by 414.9 % compared to that of original strain ZZZ816 (392 mg/L) and was significantly higher than all the other industrial HA-producing strains in published reports.
竹红菌素 A（HA）作为治疗皮肤病的最佳天然色素和生物活性剂之一，预计将在光动力疗法（PDT）中在抗癌和抗病毒治疗中发挥重要作用。在这项研究中，从毛竹（Phyllostachys edulis）种子中分离出了一株产 HA 的菌株 ZZZ816（拟青霉属），并用γ射线辐照对原始菌株的孢子进行诱变。用不同剂量（20、50、80、100、150、180、300 和 500Gy）的钴-60γ（（60）Coγ）处理后，选择 100Gy 作为最佳条件，导致孢子的致死率为 77.2%，正突变频率为 35%。通过七种检测方法的组合，对产 HA 能力最强的菌株（H-4-2）的提取物进行了精确分析，最大 HA 含量达到 2018.3mg/L。与原始菌株 ZZZ816（392mg/L）相比，H-4-2 的 HA 产量增加了 414.9%，明显高于已发表报告中的所有其他工业产 HA 菌株。
