Liang Xiao-Hui, Cai Yu-Jie, Liao Xiang-Ru, Wu Kang, Wang Lei, Zhang Da-Bing, Meng Qiang
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Lihu Road 1800, Wuxi 214122, Jiangsu, China.
Microbiol Res. 2009;164(1):9-17. doi: 10.1016/j.micres.2008.08.004. Epub 2008 Sep 21.
A new hypocrellin A-producing strain, Shiraia sp. SUPER-H168, was isolated from tissues of bamboo, Brachystachyum densiflorum. The morphology of this strain was characterized with a light microscope and a scanning electronic microscope. The mycelia, conidia, pycnidia of fungus were observed. Small pycnidia (10-20 microm in length) full of small conidia appeared on the mycelia, which were first reported in this study. The 18S rDNA region of this strain was amplified and sequenced. Then a neighbor-joining tree of 18S rDNA was constructed. According to the result of analysis, the strain SUPER-H168 was proved to belong to the genus Shiraia. Hypocrellin A was produced by solid-state fermentation, extracted by acetone, isolated by preparative RP-HPLC, and identified by RP-HPLC, ESI-MS and ultraviolet-visible absorbing scanning with diode array detection. The HA production could reach 2.02 mg/g dry solid substrate.
从密花短穗竹的组织中分离出一种新的产竹红菌素A的菌株——竹黄菌SUPER-H168。用光学显微镜和扫描电子显微镜对该菌株的形态进行了表征。观察了真菌的菌丝体、分生孢子和分生孢子器。在菌丝体上出现了充满小分生孢子的小型分生孢子器（长度为10 - 20微米），这是本研究首次报道。对该菌株的18S rDNA区域进行了扩增和测序。然后构建了18S rDNA的邻接树。根据分析结果，证明菌株SUPER-H168属于竹黄菌属。通过固态发酵生产竹红菌素A，用丙酮提取，通过制备型反相高效液相色谱分离，并通过反相高效液相色谱、电喷雾电离质谱和二极管阵列检测的紫外可见吸收扫描进行鉴定。竹红菌素A的产量可达2.02毫克/克干固体底物。
