Xia Bairong, Li Huiyan, Yang Shanshan, Liu Tianbo, Lou Ge
Department of Gynecology, the Affiliated Tumor Hospital, Harbin Medical University, 150 Haping Rd, Nangang, Harbin, Heilongjiang, 150020, China.
Department of Nursing, the Affiliated Tumor Hospital, Harbin Medical University, Harbin, 150020, China.
Tumour Biol. 2016 Jul;37(7):9157-67. doi: 10.1007/s13277-016-4805-8. Epub 2016 Jan 14.
Epithelial ovarian cancer (EOC) is a common type of gynecologic cancer, which accounts for the majority of deaths among all gynecologic malignant tumors in developed countries. A series of recent studies suggested that miR-381 might play important roles in the development of various cancer types. However, the biological function of miR-381 in EOC remains to be investigated. We examined the levels of miR-381 expression in EOC tissues and cell lines. We identified miR-381 target genes by bioinformatic prediction. We also characterized the phenotype regarding cell proliferation, cell migration, and cell invasion in EOC cells lines with altered expression levels of both miR-381 and its target gene, YY1. The expression levels of miR-381 were downregulated in EOC tissues and cell lines. Overexpression of miR-381 significantly inhibited EOC cell proliferation, migration, and invasion. Restoration of YY1 expression partially reversed the phenotype induced by miR-381 overexpression. Knockdown of miR-381 target gene, YY1, mimicked the phenotype induced by miR-381 overexpression. MiR-381 regulated EOC cell through miR-381/YY1/p53 and miR-381/YY1/Wnt signaling axis. We concluded that miR-381 inhibited EOC cell proliferation, migration, and invasion, at least in part, via suppressing YY1 expression.
上皮性卵巢癌（EOC）是一种常见的妇科癌症，在发达国家，它在所有妇科恶性肿瘤导致的死亡中占大多数。最近一系列研究表明，miR-381可能在多种癌症类型的发生发展中发挥重要作用。然而，miR-381在EOC中的生物学功能仍有待研究。我们检测了EOC组织和细胞系中miR-381的表达水平。通过生物信息学预测鉴定了miR-381的靶基因。我们还对miR-381及其靶基因YY1表达水平改变的EOC细胞系中的细胞增殖、细胞迁移和细胞侵袭的表型进行了表征。miR-381在EOC组织和细胞系中的表达水平下调。miR-381的过表达显著抑制了EOC细胞的增殖、迁移和侵袭。YY1表达的恢复部分逆转了miR-381过表达诱导的表型。敲低miR-381的靶基因YY1模拟了miR-381过表达诱导的表型。miR-381通过miR-381/YY1/p53和miR-381/YY1/Wnt信号轴调节EOC细胞。我们得出结论，miR-381至少部分通过抑制YY1的表达来抑制EOC细胞的增殖、迁移和侵袭。
