Pereira Gabriela Garrastazu, Detoni Cassia Britto, Balducci Anna Giulia, Rondelli Valeria, Colombo Paolo, Guterres Silvia Stanisçuaski, Sonvico Fabio
Faculty the Pharmacy, University Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Biopharmanet-TEC, Interdepartmental Centre for Innovation in Health Products, University of Parma, Italy; Department of Pharmacy, University of Parma, Italy.
Eur J Pharm Sci. 2016 Feb 15;83:203-11. doi: 10.1016/j.ejps.2016.01.002. Epub 2016 Jan 6.
Lecithin and hyaluronic acid were used for the preparation of polysaccharide decorated nanoparticles loaded with vitamin E using the cationic lipid dioctadecyldimethylammonium bromide (DODMA). Nanoparticles showed mean particle size in the range 130-350 nm and narrow size distribution. Vitamin E encapsulation efficiency was higher than 99%. These nanoparticles were incorporated in polymeric films containing Aloe vera extract, hyaluronic acid, sodium alginate, polyethyleneoxide (PEO) and polyvinylalcohol (PVA) as an innovative treatment in skin wounds. Films were thin, flexible, resistant and suitable for application on burn wounds. Additionally, in vitro occlusion study highlighted the dependence of the occlusive effect on the presence of nanoparticles. The results obtained show that the bioadhesive films containing vitamin E acetate and Aloe vera could be an innovative therapeutic system for the treatment of skin wounds, such as burns. The controlled release of the vitamin along with a reduction in water loss through damaged skin provided by the nanoparticle-loaded polymer film are considered important features for an improvement in wound healing and skin regeneration.
使用阳离子脂质二辛基二甲基溴化铵（DODMA），将卵磷脂和透明质酸用于制备负载维生素E的多糖修饰纳米颗粒。纳米颗粒的平均粒径在130 - 350 nm范围内，粒径分布窄。维生素E的包封率高于99%。这些纳米颗粒被掺入含有芦荟提取物、透明质酸、海藻酸钠、聚环氧乙烷（PEO）和聚乙烯醇（PVA）的聚合物薄膜中，作为皮肤伤口的一种创新治疗方法。薄膜薄、柔韧、耐用，适用于烧伤伤口。此外，体外封闭性研究突出了封闭效果对纳米颗粒存在的依赖性。所获得的结果表明，含有维生素E醋酸酯和芦荟的生物粘附薄膜可能是一种用于治疗皮肤伤口（如烧伤）的创新治疗系统。纳米颗粒负载聚合物薄膜提供的维生素的控释以及通过受损皮肤的水分流失减少，被认为是改善伤口愈合和皮肤再生的重要特征。
