Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
Department of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading RG6 6AD, UK.
Food Chem. 2016 May 1;198:85-92. doi: 10.1016/j.foodchem.2015.11.031. Epub 2015 Nov 11.
Crude enzymes produced via solid state fermentation (SSF) using wheat milling by-products have been employed for both fermentation media production using flour-rich waste (FRW) streams and lysis of Rhodosporidium toruloides yeast cells. Filter sterilization of crude hydrolysates was more beneficial than heat sterilization regarding yeast growth and microbial oil production. The initial carbon to free amino nitrogen ratio of crude hydrolysates was optimised (80.2g/g) in fed-batch cultures of R. toruloides leading to a total dry weight of 61.2g/L with microbial oil content of 61.8% (w/w). Employing a feeding strategy where the glucose concentration was maintained in the range of 12.2-17.6g/L led to the highest productivity (0.32 g/L·h). The crude enzymes produced by SSF were utilised for yeast cell treatment leading to simultaneous release of around 80% of total lipids in the broth and production of a hydrolysate suitable as yeast extract replacement.
采用固态发酵（SSF）生产的粗酶制剂，利用小麦加工副产物，可用于发酵培养基生产和 Rhodosporidium toruloides 酵母细胞裂解。与热灭菌相比，粗水解液的过滤灭菌更有利于酵母生长和微生物油生产。在 Rhodosporidium toruloides 的分批补料培养中，优化了粗水解物的初始碳与游离氨基酸氮比（80.2g/g），最终总干重达到 61.2g/L，微生物油含量为 61.8%（w/w）。采用葡萄糖浓度维持在 12.2-17.6g/L 范围内的进料策略，可获得最高的生产力（0.32 g/L·h）。SSF 生产的粗酶制剂用于酵母细胞处理，可同时释放发酵液中约 80%的总脂，并生产出适合替代酵母提取物的水解液。
