Arnone James T
Department of Biology, William Paterson University, Wayne, NJ 07470, USA.
Microorganisms. 2020 Feb 26;8(3):321. doi: 10.3390/microorganisms8030321.
The growing global population and developing world has put a strain on non-renewable natural resources, such as fuels. The shift to renewable sources will, thus, help meet demands, often through the modification of existing biosynthetic pathways or the introduction of novel pathways into non-native species. There are several useful biosynthetic pathways endogenous to organisms that are not conducive for the scale-up necessary for industrial use. The use of genetic and synthetic biological approaches to engineer these pathways in non-native organisms can help ameliorate these challenges. The budding yeast offers several advantages for genetic engineering for this purpose due to its widespread use as a model system studied by many researchers. The focus of this review is to present a primer on understanding genomic considerations prior to genetic modification and manipulation of . The choice of a site for genetic manipulation can have broad implications on transcription throughout a region and this review will present the current understanding of position effects on transcription.
全球人口的增长以及发展中世界给不可再生自然资源（如燃料）带来了压力。因此，向可再生能源的转变通常通过改造现有的生物合成途径或将新途径引入非本地物种来帮助满足需求。生物体中存在几种有用的内源性生物合成途径，但不利于扩大到工业使用所需的规模。利用基因和合成生物学方法在非本地生物体中改造这些途径有助于缓解这些挑战。由于许多研究人员广泛将其用作模型系统，出芽酵母为此目的的基因工程提供了几个优势。本综述的重点是在对[具体对象]进行基因改造和操作之前，提供一篇关于理解基因组考量因素的入门文章。基因操作位点的选择可能会对整个区域的转录产生广泛影响，本综述将介绍目前对转录位置效应的理解。
