Department of Physical Chemistry and Applied Thermodynamics, EPS, Edificio Leonardo da Vinci, Campus de Rabanales, Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario ceiA3, 14071 Córdoba, Spain.
Department of Food Science and Technology, Ionian University, Argostoli 28100, Kefalonia, Greece.
Bioresour Technol. 2021 Mar;323:124597. doi: 10.1016/j.biortech.2020.124597. Epub 2020 Dec 24.
Biodiesel production using microbial oil derived from food waste discarded by the hospilatity sector could provide a sustainable replacement for diesel fuel. Discarded potato peels were used in solid-state fermentations of Aspergillus awamori for the production of glucoamylase (30 U/g) and protease (50 U/g). Hospitality food waste hydrolysis led to 98% (w/w) starch to glucose conversion yields. Crude hydrolyzates were used in shake flask fermentations with the oleaginous yeast Rhodosporidium toruloides Y-27012 leading to 32.9 g/L total dry weight (TDW) with 36.4% (w/w) intracellular lipid content. Fed-bath bioreactor cultures resulted in TDW of 53.9 g/L and lipid concentration of 26.7 g/L. Principal component analysis showed a fatty acid profile similar to soybean oil and solid food waste oil. Microbial oil was transesterified into biodiesel with satisfactory performance considering the European standard EN 14214. This work demonstrated that valorization of food waste for biodiesel production is feasible.
利用餐饮业丢弃的食物废料生产微生物油来生产生物柴油,可以为柴油燃料提供可持续的替代品。废弃的土豆皮被用于固态发酵米曲霉生产葡萄糖淀粉酶(30 U/g)和蛋白酶(50 U/g)。餐饮食物废料的水解导致 98%(w/w)的淀粉转化为葡萄糖。粗水解产物在摇瓶发酵中与油脂酵母 Rhodosporidium toruloides Y-27012 一起使用,导致总干重(TDW)为 32.9 g/L,细胞内脂质含量为 36.4%(w/w)。补料分批生物反应器培养的 TDW 为 53.9 g/L,脂质浓度为 26.7 g/L。主成分分析显示脂肪酸谱类似于大豆油和固体食物废料油。根据欧洲标准 EN 14214,微生物油经酯交换转化为生物柴油,性能令人满意。这项工作表明,对食物废料进行生物柴油生产的增值是可行的。