Legault Jean, Girard-Lalancette Karl, Dufour Dominic, Pichette André
Laboratoire d'analyse et de séparation des essences végétales (LASEVE), Département des Sciences fondamentales, Université du Québec à Chicoutimi (UQAC), 555, boulevard de l'Université, Chicoutimi, Québec G7H 2B1, Canada.
Antioxidants (Basel). 2013 Jul 11;2(3):77-89. doi: 10.3390/antiox2030077.
The bark of boreal forest conifers has been traditionally used by Native Americans to treat various ailments and diseases. Some of these diseases involve reactive oxygen species (ROS) that can be prevented by the consumption of antioxidants such as phenolic compounds that can be found in medicinal plants. In this study, ultrasonic assisted extraction has been performed under various solvent conditions (water:ethanol mixtures) on the bark of seven boreal forest conifers used by Native Americans including: Pinus strobus, Pinus resinosa, Pinus banksiana, Picea mariana, Picea glauca, Larix laricina, and Abies balsamea. The total phenolic content, as well as ORACFL potency and cellular antioxidant activity (IC50), were evaluated for all bark extracts, and compared with the standardized water extract of Pinus maritima bark (Pycnogenol), which showed clinical efficiency to prevent ROS deleterious effects. The best overall phenolic extraction yield and antioxidant potential was obtained with Picea glauca and Picea mariana. Interestingly, total phenolic content of these bark extracts was similar to Pycnogenol but their antioxidant activity were higher. Moreover, most of the extracts did not inhibit the growth of human skin fibroblasts, WS1. A significant correlation was found between the total phenolic content and the antioxidant activity for water extracts suggesting that these compounds are involved in the activity.
北方针叶林针叶树的树皮传统上被美洲原住民用于治疗各种疾病。其中一些疾病涉及活性氧（ROS），而食用抗氧化剂（如药用植物中含有的酚类化合物）可以预防这些疾病。在本研究中，在各种溶剂条件（水：乙醇混合物）下，对美洲原住民使用的七种北方针叶林针叶树的树皮进行了超声辅助提取，这些针叶树包括：美国白松、黑云杉、班克松、黑云杉、白云杉、落叶松和香脂冷杉。对所有树皮提取物的总酚含量、ORACFL效价和细胞抗氧化活性（IC50）进行了评估，并与滨海松树皮的标准化水提取物（碧萝芷）进行了比较，后者已显示出预防ROS有害作用的临床效果。白云杉和黑云杉的总酚提取率和抗氧化潜力总体最佳。有趣的是，这些树皮提取物的总酚含量与碧萝芷相似，但其抗氧化活性更高。此外，大多数提取物不会抑制人皮肤成纤维细胞WS1的生长。水提取物的总酚含量与抗氧化活性之间存在显著相关性，表明这些化合物参与了该活性。
