Department of Forest Products and Biotechnology, College of Science and Technology, Kookmin University, 861-1 Chongnung-dong, Songbuk-gu, Seoul, 136-702, Republic of Korea.
Biomed Res Int. 2019 Jul 29;2019:3520675. doi: 10.1155/2019/3520675. eCollection 2019.
It is well established that various extraction factors, including the method, temperature, time, and solvent system, significantly influence the antioxidant quality of plant-derived products. Previously, we observed that extraction of bark (PDB) by the most common traditional Soxhlet method using water at two different temperature conditions 60°C and 100°C for 6-15 h noticeably altered their antioxidant quality. In this study, we examined the impact of different extraction solvents such as ethanol, methanol, isopropanol, acetonitrile, and acetone at a different percentage with water (vol/vol) on antioxidant efficiency as well as the total phenolic content (TPC) of PDB extracts. Among the fourteen different PDB extracts, the extracts obtained from 20% ethanol (E20), 40% ethanol (E40), and 20% acetonitrile (ACN20) showed more significant antioxidant potential, as well as high total phenol content (TPC). Extracts from other aqueous mixtures of organic solvents such as isopropanol, acetone, and methanol, as well as water, showed lesser antioxidant capacity and also had less TPC compared to these three most active extracts, E20, E40, and ACN20. Moreover, using ethanol at 100% for extraction significantly decreased the TPC and antioxidant capacity of PDB extracts. Data are implicating that an increased phenolic content in PDB extracts proportionally increases their antioxidant efficiency.
已证实，各种提取因素，包括方法、温度、时间和溶剂系统，会显著影响植物源性产品的抗氧化质量。此前，我们观察到，用最常见的传统索氏提取法，分别以水在 60°C 和 100°C 两种不同温度条件下提取 6-15 小时，树皮（PDB）的抗氧化质量会明显改变。在这项研究中，我们研究了不同提取溶剂（如乙醇、甲醇、异丙醇、乙腈和丙酮）以不同浓度与水（体积/体积）混合，对 PDB 提取物抗氧化效率和总酚含量（TPC）的影响。在 14 种不同的 PDB 提取物中，从 20%乙醇（E20）、40%乙醇（E40）和 20%乙腈（ACN20）获得的提取物具有更显著的抗氧化潜力，以及高总酚含量（TPC）。其他含有有机溶剂（如异丙醇、丙酮和甲醇）的水相混合物的提取物，以及水，其抗氧化能力较小，TPC 也比这三种最有效提取物（E20、E40 和 ACN20）少。此外，用 100%乙醇进行提取会显著降低 PDB 提取物的 TPC 和抗氧化能力。数据表明，PDB 提取物中酚类含量的增加与抗氧化效率成正比。
