维生素 D 受体基因多态性与妊娠期糖尿病的关联：一项中国武汉的病例对照研究。
Association of vitamin D receptor gene polymorphisms with gestational diabetes mellitus-a case control study in Wuhan, China.
机构信息
Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, No.745 Wuluo Road, Wuhan, China.
Department of non-communicable chronic disease, Wuhan Centers for Disease Prevention and Control, Wuhan, China.
出版信息
BMC Pregnancy Childbirth. 2021 Feb 17;21(1):142. doi: 10.1186/s12884-021-03621-y.
BACKGROUND
Gestational diabetes mellitus (GDM) increased risk of perinatal complications for both the women and the fetuses. The association between the vitamin D receptor (VDR) gene polymorphism and GDM has not been thoroughly investigated in Chinese pregnant women. Therefore, we aimed to determine whether VDR gene single nucleotide polymorphisms (SNPs) rs154410, rs7975232, rs731236, rs2228570 and rs739837 contribute to GDM risk in Wuhan, China. Moreover, we aimed to explore their combined effects on the risk of GDM.
METHODS
Pregnant women who had prenatal examinations at 24 to 28 weeks' gestation in our hospital from January 15, 2018 to March 31, 2019 were included in this case-control study. After exclusion, a total of 1684 pregnant women (826 GDM patients and 858 non-diabetic controls) were recruited. The clinical information and blood samples were collected by trained interviewers and nurses. Genotyping of candidate SNPs was conducted on the Sequenom MassARRAY platform. Statistical analyses including t-test, ANOVA, chi-square test and logistic regression were performed to the data with SPSS Software to evaluate differences in genotype distribution and associations with GDM risk. Multifactor dimensionality reduction method was used to explore the gene-gene interactions on the risk of GDM.
RESULTS
Differences in age, pre-pregnancy BMI, family history of diabetes and previous history of GDM between the case and control groups were statistically significant (P < 0.05), whereas no significant differences were found in height, gravidity, parity, and age of menarche (P > 0.05). There were no significant differences at genotype distributions of the examined VDR gene SNPs (P > 0.05). After adjusting by age, pre-pregnancy BMI, family history of diabetes, the results of logistic regression analysis showed no associations of the five SNPs with GDM in all the four genotype models(P > 0.05). Furthermore, there were no gene-gene interactions on the GDM risk among the five examined VDR gene SNPs.
CONCLUSIONS
The VDR gene SNPs rs154410, rs7975232, rs731236, rs2228570 and rs739837 showed neither significant associations nor gene-gene interactions with GDM in Wuhan, China.
背景
妊娠糖尿病（GDM）会增加母婴围产期并发症的风险。维生素 D 受体（VDR）基因多态性与 GDM 之间的关系尚未在中国孕妇中进行深入研究。因此，我们旨在确定 VDR 基因单核苷酸多态性（SNP）rs154410、rs7975232、rs731236、rs2228570 和 rs739837 是否与中国武汉的 GDM 风险相关。此外，我们旨在探讨它们对 GDM 风险的联合作用。
方法
本病例对照研究纳入了 2018 年 1 月 15 日至 2019 年 3 月 31 日在我院进行 24-28 周产前检查的孕妇。排除后，共纳入 1684 名孕妇（826 名 GDM 患者和 858 名非糖尿病对照）。通过培训过的访谈者和护士收集临床信息和血样。候选 SNP 的基因分型在 Sequenom MassARRAY 平台上进行。使用 SPSS 软件进行包括 t 检验、方差分析、卡方检验和 logistic 回归在内的统计分析，以评估基因型分布的差异和与 GDM 风险的关联。多因素维度缩减方法用于探讨基因-基因相互作用对 GDM 风险的影响。
结果
病例组和对照组在年龄、孕前 BMI、糖尿病家族史和既往 GDM 史方面的差异有统计学意义（P<0.05），而身高、孕次、产次和初潮年龄差异无统计学意义（P>0.05）。所检查的 VDR 基因 SNP 的基因型分布无显著差异（P>0.05）。经年龄、孕前 BMI、糖尿病家族史校正后，logistic 回归分析结果显示，五种 SNP 在所有四种基因型模型中与 GDM 均无关联（P>0.05）。此外，在五个被检查的 VDR 基因 SNP 中，没有发现基因-基因相互作用与 GDM 风险相关。
结论
在中国武汉，VDR 基因 SNPs rs154410、rs7975232、rs731236、rs2228570 和 rs739837 与 GDM 无显著关联，也无基因-基因相互作用。
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