Department of Chemistry, University of California, 1120 Natural Sciences II, Irvine, CA, 92697, USA.
Department of Molecular Biology and Biochemistry, University of California, 3205 McGaugh Hall, Irvine, CA, 92697, USA.
Chembiochem. 2018 Mar 2;19(5):470-477. doi: 10.1002/cbic.201700542. Epub 2018 Jan 31.
New applications for bioluminescence imaging require an expanded set of luciferase enzymes and luciferin substrates. Here, we report two novel luciferins for use in vitro and in cells. These molecules comprise regioisomeric pyridone cores that can be accessed from a common synthetic route. The analogues exhibited unique emission spectra with firefly luciferase, although photon intensities remained weak. Enhanced light outputs were achieved by using mutant luciferase enzymes. One of the luciferin-luciferase pairs produced light on par with native probes in live cells. The pyridone analogues and complementary luciferases add to a growing set of designer probes for bioluminescence imaging.
新的生物发光成像应用需要扩展一套新的荧光素酶和荧光素底物。在这里，我们报告了两种新的用于体外和细胞内的荧光素。这些分子包含可以从共同的合成途径获得的区域异构吡喃酮核心。尽管光子强度仍然较弱，但这些类似物与萤火虫荧光素酶表现出独特的发射光谱。通过使用突变荧光素酶可以实现增强的光输出。在活细胞中，其中一对荧光素-荧光素酶产生的光与天然探针相当。吡喃酮类似物和互补的荧光素酶为生物发光成像的设计探针集增添了新成员。
