Sun Qi, Yang Shu-Hou, Wu Lei, Yang Wen-Chao, Yang Guang-Fu
Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University , Wuhan 430079, P.R. China.
Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 30071, P.R. China.
Anal Chem. 2016 Feb 16;88(4):2266-72. doi: 10.1021/acs.analchem.5b04029. Epub 2016 Feb 1.
A benzoquinolizine coumarin-based fluorescent probe was developed for detecting thiophenols, demonstrating the superior fluorescence properties caused by the decay of the twisting effect of N,N-diethylamino group of coumarin. It discriminated thiophenols from various analytes including aliphatic thiols with good selectivity and displayed ∼700-fold fluorescence intensity enhancement and a remarkable limit of detection (4.5 nM). The new probe also can be applied to quantitative determine the concentrations of thiophenol in water samples and living cells.
开发了一种基于苯并喹嗪香豆素的荧光探针用于检测硫酚，该探针展示出由于香豆素的N，N - 二乙氨基扭转效应衰减而产生的优异荧光特性。它能以良好的选择性将硫酚与包括脂肪族硫醇在内的各种分析物区分开来，荧光强度增强约700倍，检测限低至4.5 nM。这种新型探针还可用于定量测定水样和活细胞中硫酚的浓度。
