Borucki Monica K, Lao Victoria, Hwang Mona, Gardner Shea, Adney Danielle, Munster Vincent, Bowen Richard, Allen Jonathan E
Lawrence Livermore National Laboratory, Livermore, California, United States of America.
Colorado State University, Fort Collins, Colorado, United States of America.
PLoS One. 2016 Jan 20;11(1):e0146251. doi: 10.1371/journal.pone.0146251. eCollection 2016.
Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging human pathogen related to SARS virus. In vitro studies indicate this virus may have a broad host range suggesting an increased pandemic potential. Genetic and epidemiological evidence indicate camels serve as a reservoir for MERS virus but the mechanism of cross species transmission is unclear and many questions remain regarding the susceptibility of humans to infection. Deep sequencing data was obtained from the nasal samples of three camels that had been experimentally infected with a human MERS-CoV isolate. A majority of the genome was covered and average coverage was greater than 12,000x depth. Although only 5 mutations were detected in the consensus sequences, 473 intrahost single nucleotide variants were identified. Many of these variants were present at high frequencies and could potentially influence viral phenotype and the sensitivity of detection assays that target these regions for primer or probe binding.
中东呼吸综合征冠状病毒（MERS-CoV）是一种与SARS病毒相关的新型人类病原体。体外研究表明，这种病毒可能具有广泛的宿主范围，提示其大流行潜力增加。遗传学和流行病学证据表明，骆驼是MERS病毒的宿主，但跨物种传播机制尚不清楚，关于人类对感染的易感性仍有许多问题。从三只经实验感染人MERS-CoV分离株的骆驼的鼻腔样本中获得了深度测序数据。大部分基因组被覆盖，平均覆盖深度大于12000倍。虽然在共有序列中仅检测到5个突变，但鉴定出了473个宿主内单核苷酸变异。其中许多变异以高频率存在，并可能潜在地影响病毒表型以及针对这些区域进行引物或探针结合的检测分析的灵敏度。
