Barcat L, Dekens C, Caron-Lesenechal E, Degorre C, Cauliez A, Riou B, Vasseur A, Gromada V, Leke A, Bach V, Tourneux P
Service de réanimation pédiatrique, pôle femme-couple-enfant, CHU d'Amiens, 80054 Amiens cedex 1, France; PériTox (UMI 01), faculté de médecine, université Picardie - Jules-Verne, 80000 Amiens, France.
Service de réanimation pédiatrique, pôle femme-couple-enfant, CHU d'Amiens, 80054 Amiens cedex 1, France.
Arch Pediatr. 2016 Mar;23(3):255-60. doi: 10.1016/j.arcped.2015.12.003. Epub 2016 Jan 12.
Hemoglobin (Hb) measurement is essential for the monitoring of anemia in preterm neonates to assess if any bleeding (pulmonary, cerebral, digestive) is present. EDTA samples require 500 μL vs. 10 μL for the Hemocue(®) system. This system has been evaluated and validated in adults and children but not in preterm neonates with fetal hemoglobin. The aim of the study was to compare Hb measurement with the Hemocue(®) system vs. the EDTA laboratory system on fetal Hb in preterm neonates.
This was a prospective study conducted in the preterm intensive care unit in the Amiens Hospital. Preterm neonates, before 28 days of life, requiring EDTA (Hb) measurement were included. Two Hemocues(®) were performed at the same time. Postnatal age (correlated to the fetal hemoglobin level decrease), blood sample site, and other factors that could influence the Hb result were evaluated.
Seventy-six EDTA and 152 Hemocue(®) samples from 38 preterm neonates were included. The term was 28.1±3.7 weeks of gestation, the birth weight was 1215.5±657 g. We found a good correlation between the Hemocue(®) and EDTA samples (Hemocue(®)=EDTA*0.94-0.4; R(2)=0.63; P<0.001). The influence of confounding factors was insignificant.
The use of the Hemocue(®) system showed a good correlation with the EDTA measurement of fetal Hb, with a moderate bias (-0.2±1.5 g/dL), which remained stable for the first 28 days of life.
血红蛋白（Hb）测量对于监测早产儿贫血以评估是否存在任何出血（肺部、脑部、消化道）至关重要。乙二胺四乙酸（EDTA）样本需要500微升，而Hemocue®系统仅需10微升。该系统已在成人和儿童中进行了评估和验证，但尚未在患有胎儿血红蛋白的早产儿中进行。本研究的目的是比较Hemocue®系统与EDTA实验室系统对早产儿胎儿血红蛋白的测量结果。
这是一项在亚眠医院早产儿重症监护病房进行的前瞻性研究。纳入出生28天内需要进行EDTA（Hb）测量的早产儿。同时进行两次Hemocue®测量。评估出生后年龄（与胎儿血红蛋白水平下降相关）、血样采集部位以及其他可能影响Hb结果的因素。
纳入了38例早产儿的76份EDTA样本和152份Hemocue®样本。孕周为28.1±3.7周，出生体重为1215.5±657克。我们发现Hemocue®样本与EDTA样本之间具有良好的相关性（Hemocue® = EDTA * 0.94 - 0.4；R² = 0.63；P < 0.001）。混杂因素的影响不显著。
使用Hemocue®系统与胎儿Hb的EDTA测量结果显示出良好的相关性，偏差适中（-0.2±1.5克/分升），在出生后的前28天内保持稳定。
