Hu Jie, Leng Xuan, Hu Yijun, Atik Alp, Song Xin, Li Zhixi, Liu Yuhua, Lu Lin
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
Royal Victorian Eye and Ear Hospital, Melbourne, Australia.
PLoS One. 2016 Jan 22;11(1):e0147346. doi: 10.1371/journal.pone.0147346. eCollection 2016.
To investigate the cytokine concentrations in the aqueous humor of patients with refractory polypoidal choroidal vasculopathy (PCV).
Three separate groups of patients were studied-refractory PCV (Group A, 41 eyes), stable PCV (Group B, 39 eyes) and senile cataract (Group C, 44 eyes). Aqueous humor samples were collected at two time points for Groups A and B-before the first intravitreal ranibizumab injection and before the last injection. Aqueous humor samples were collected prior to phacoemulsification in Group C. The cytokine concentrations of interleukin 2, 6, and 8 (IL-2, IL-6, and IL-8), tumor necrosis factor α (TNF-α), monocyte chemotactic protein 1 (MCP-1), and vascular endothelial growth factor (VEGF) were measured by cytometric bead array and flow cytometry.
Before the first treatment, the MCP-1, VEGF, and TNF-α levels in Group A were significantly higher than those in Group C (P < 0.05), and the MCP-1 and VEGF levels in Group A were significantly higher than those in Group B (P < 0.05). Significantly higher MCP-1 and VEGF levels were seen in Group B compared to Group C (P < 0.05). Before the final treatment, the MCP-1, VEGF, and TNF-α concentrations in Group A were significantly higher than those in Group B (P < 0.05) and Group C (P < 0.05). IL-2 levels were significantly lower in Group A compared to Group B (P < 0.05) and Group C (P < 0.05).
Inflammatory cytokines such as MCP-1, VEGF, and TNF-α may be associated with the pathogenesis of both stable and refractory PCV.
研究难治性息肉状脉络膜血管病变（PCV）患者房水中细胞因子的浓度。
对三组不同患者进行研究——难治性PCV患者（A组，41只眼）、稳定期PCV患者（B组，39只眼）和老年性白内障患者（C组，44只眼）。A组和B组在两个时间点采集房水样本——第一次玻璃体内注射雷珠单抗前和最后一次注射前。C组在白内障超声乳化术前采集房水样本。采用细胞计数珠阵列和流式细胞术检测白细胞介素2、6和8（IL-2、IL-6和IL-8）、肿瘤坏死因子α（TNF-α）、单核细胞趋化蛋白1（MCP-1）和血管内皮生长因子（VEGF）的细胞因子浓度。
首次治疗前，A组的MCP-1、VEGF和TNF-α水平显著高于C组（P<0.05），且A组的MCP-1和VEGF水平显著高于B组（P<0.05）。B组的MCP-1和VEGF水平显著高于C组（P<0.05）。末次治疗前，A组的MCP-1、VEGF和TNF-α浓度显著高于B组（P<0.05）和C组（P<0.05）。A组的IL-2水平显著低于B组（P<0.05）和C组（P<0.05）。
MCP-1、VEGF和TNF-α等炎性细胞因子可能与稳定期和难治性PCV的发病机制有关。
