Supong Khomsan, Thawai Chitti, Choowong Wilunda, Kittiwongwattana Chokchai, Thanaboripat Dusanee, Laosinwattana Chamroon, Koohakan Prommart, Parinthawong Nonglak, Pittayakhajonwut Pattama
Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Ladkrabang, Bangkok 10520, Thailand; Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Ladkrabang, Bangkok 10520, Thailand; Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Res Microbiol. 2016 May;167(4):290-298. doi: 10.1016/j.resmic.2016.01.004. Epub 2016 Jan 22.
An endophytic actinomycete strain BCC72023 was isolated from rice (Oryza sativa L.) and identified as the genus Streptomyces, based on phenotypic, chemotaxonomic and 16S rRNA gene sequence analyses. The strain showed 99.80% similarity compared with Streptomyces samsunensis M1463(T). Chemical investigation led to the isolation of three macrolides, efomycins M (1), G (2) and oxohygrolidin (3), along with two polyethers, abierixin (4) and 29-O-methylabierixin (5). To our knowledge, this is the first report of efomycin M being isolated from a natural source. The compounds were identified using spectroscopic techniques and comparison with previously published data. All compounds exhibited antimalarial activity against the Plasmodium falciparum, K-1 strain, a multidrug-resistant strain, with IC50 values in a range of 1.40-5.23 μg/ml. In addition, these compounds were evaluated for biological activity against Mycobacterium tuberculosis, Bacillus cereus, Colletotrichum gloeosporioides and Colletotrichum capsici, as well as cytotoxicity against both cancerous (MCF-7, KB, NCI-H187) and non-cancerous (Vero) cells.
从水稻（Oryza sativa L.）中分离出一株内生放线菌菌株BCC72023，基于表型、化学分类学和16S rRNA基因序列分析，鉴定为链霉菌属。该菌株与萨姆松链霉菌M1463(T)的相似度为99.80%。化学研究导致分离出三种大环内酯类化合物，即埃弗霉素M（1）、G（2）和氧代 Hygrolidin（3），以及两种聚醚类化合物，阿比瑞辛（4）和29-O-甲基阿比瑞辛（5）。据我们所知，这是首次从天然来源分离出埃弗霉素M的报道。这些化合物通过光谱技术并与先前发表的数据进行比较来鉴定。所有化合物对恶性疟原虫K-1株（一种多药耐药菌株）均表现出抗疟活性，IC50值在1.40 - 5.23μg/ml范围内。此外，还评估了这些化合物对结核分枝杆菌、蜡样芽孢杆菌、胶孢炭疽菌和辣椒炭疽菌的生物活性，以及对癌细胞（MCF-7、KB、NCI-H187）和非癌细胞（Vero）的细胞毒性。
