Eroğlu Canan, Seçme Mücahit, Atmaca Pelin, Kaygusuz Oğuzhan, Gezer Kutret, Bağcı Gülseren, Dodurga Yavuz
Department of Medical Biology, Faculty of Medicine, Pamukkale University, Kınıklı/Denizli, Turkey.
Department of Biology, Faculty of Science, Pamukkale University, Denizli, Turkey.
Cytotechnology. 2016 Oct;68(5):2075-81. doi: 10.1007/s10616-016-9947-4. Epub 2016 Jan 28.
In this study, in order to investigate the anticancer mechanism of Calvatia gigantea extract, edible mushroom species, which belong to Lycoperdaceae family, changes of CCND1, CCND2, CDK4, p21, Akt, Bax, Bcl-2, p53, caspase-3 and caspase-9 were evaluated in A549 lung cancer cells. Cytotoxic effect of C. gigantea extract was evaluated by using XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5 carboxanilide). The C. gigantea extract was treated in a time and dose dependent manner within the range 25 μg/ml-2 mg/ml to determine the IC50 dose. IC50 dose for C. gigantea extract was detected as 500 μg/ml for 72 h. According to expression results, while CCND1, CCND2, CDK4, Akt and Bcl-2 expression clearly decreased, Bax, p53, caspase-3 and caspase-9 expression clearly increased in the dose group cells (A549 cells treated with 500 μg/ml dose of C. gigantea extract for 72 h). However, there was no change in p21 expression. C. gigantea extract induced cell cycle arrest and apoptosis by decreasing the CCND1, CCND2, CDK4, Akt and Bcl-2 expression and by increasing Bax, p53, caspase-3 and caspase-9 expression in A549 cells. Mushrooms are eukaryotic organisms heavily used because of their supposedly anticancer effect. Many mushroom species have been used for medical purposes, as a result of also having many effects such as antibiotic, antiviral and anticancer effects. It is thought that the C. gigantea extract may be a significant agent for treatment of lung cancer as a single agent or in combination with other drugs.
在本研究中，为了探究马勃提取物（一种属于马勃科的可食用蘑菇物种）的抗癌机制，对A549肺癌细胞中CCND1、CCND2、CDK4、p21、Akt、Bax、Bcl - 2、p53、caspase - 3和caspase - 9的变化进行了评估。通过使用XTT（2,3 - 双 -（2 - 甲氧基 - 4 - 硝基 - 5 - 磺基苯基）- 2H - 四唑 - 5 - 羧苯胺）评估马勃提取物的细胞毒性作用。马勃提取物在25μg/ml至2mg/ml的范围内以时间和剂量依赖性方式处理，以确定IC50剂量。马勃提取物的IC50剂量在72小时时检测为500μg/ml。根据表达结果，在剂量组细胞（用500μg/ml剂量的马勃提取物处理72小时的A549细胞）中，CCND1、CCND2、CDK4、Akt和Bcl - 2的表达明显降低，而Bax、p53、caspase - 3和caspase - 9的表达明显增加。然而，p21的表达没有变化。马勃提取物通过降低A549细胞中CCND1、CCND2、CDK4、Akt和Bcl - 2的表达以及增加Bax、p53、caspase - 3和caspase - 9的表达来诱导细胞周期停滞和凋亡。蘑菇是由于其所谓的抗癌作用而被大量使用的真核生物。许多蘑菇物种由于还具有抗生素、抗病毒和抗癌等多种作用而被用于医疗目的。人们认为马勃提取物可能作为单一药物或与其他药物联合使用，成为治疗肺癌的重要药物。
