Li Changyou, Wu Xiao, Sun Rongli, Zhao Peng, Liu Fengjuan, Zhang Chunling
Key Laboratory of Cancer Therapy, Qingdao Central Hospital, No.127 Siliu South Road, Qingdao, Shandong 266042, P.R.China. Email:
Asian Pac J Cancer Prev. 2016 Nov 1;17(11):4893-4898. doi: 10.22034/APJCP.2016.17.11.4893.
Objective: To investigate the impact of a Croton tiglium extract on cellular proliferation and apoptosis in a nonsmall cell lung cancer cell line (A549) in vitro. Methods: A Croton tiglium seed methanol extract was prepare and assessed for effects on A549 cells regarding cellular proliferation, apoptotic rates, and expression of apoptosis related genes and proteins using real-time PCR and immunofluorescence. Results: The tested Croton tiglium extract inhibited A549 cell proliferation in a dose- and time-dependent manner, with significant elevation of apoptotic indexes at various concentrations after 24 h. In addition, rates in both early and late stages were higher in treated than untreated groups, the 100 μg/ml dose causing the highest levels of apoptosis. RT-PCR showed that A549 cells treated with 100 μg/ml Croton tiglium extract for 24 h has markedly higher Bax mRNA expression levels and obviously lower Bcl-2 expression levels than controls, equivalent results being observed for proteins by immunofluorescence. However, the mRNA expression levels of Fas and caspase-8 were not significantly altered. Conclusion: A Croton tiglium extract can inhibit proliferation of A549 cells and promote apoptosis though Bax/Bcl-2 pathways.
探讨巴豆提取物对体外非小细胞肺癌细胞系（A549）细胞增殖和凋亡的影响。方法：制备巴豆种子甲醇提取物，并使用实时荧光定量PCR和免疫荧光法评估其对A549细胞的细胞增殖、凋亡率以及凋亡相关基因和蛋白表达的影响。结果：受试巴豆提取物以剂量和时间依赖性方式抑制A549细胞增殖，24小时后不同浓度下凋亡指数显著升高。此外，处理组的早期和晚期凋亡率均高于未处理组，100μg/ml剂量导致最高水平的凋亡。逆转录-聚合酶链反应（RT-PCR）显示，用100μg/ml巴豆提取物处理24小时的A549细胞，其Bax mRNA表达水平明显高于对照组，而Bcl-2表达水平明显低于对照组，免疫荧光检测蛋白质得到了相同的结果。然而，Fas和半胱天冬酶-8的mRNA表达水平没有显著改变。结论：巴豆提取物可通过Bax/Bcl-2途径抑制A549细胞增殖并促进凋亡。
