Zheng Xianjin, Wang Dan, Ning Kang, Liang Te, Wang Minghang, Jiang Meng, Zhang Dabing
Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Virus Res. 2016 Apr 2;215:37-41. doi: 10.1016/j.virusres.2016.01.020. Epub 2016 Jan 30.
A novel strain of duck reovirus (DRV) associated with a high mortality in Pekin ducklings in China, 2013, was isolated and characterized. This strain (designated as HN5d) grew well in Vero cells and produced marked cytopathic effects. HN5d contains 10 dsRNA genome segments, a typical feature of avian orthoreovirus. Following cloning, sequencing, and sequence analysis of the genome segments, a unique deletion of 18 amino acids was found in the sigma C protein of HN5d when compared with that of the recent Chinese waterfowl reoviruses (e.g., DRV 091). Phylogenetic analysis of cDNA amplicons of segments encoding for the outer capsid proteins revealed that HN5d is a novel genotype 2 waterfowl reovirus isolate. Inoculation of Pekin ducklings with HN5d resulted in splenic necrosis, a typical feature of "Duck spleen necrosis disease" (DSND) discovered in China in 2006. Unlike the typical DSND, HN5d produced severer hemorrhagic and/or necrotic lesions in livers of experimentally infected ducklings. 20-30% of death was observed during the first 7 day in the experimentally exposed birds. These findings suggest that HN5d is a novel duck reovirus isolate with severer pathogenicity in Pekin ducklings.
2013年，在中国一种与北京鸭雏鸭高死亡率相关的新型鸭呼肠孤病毒（DRV）被分离并鉴定。该毒株（命名为HN5d）在Vero细胞中生长良好并产生明显的细胞病变效应。HN5d含有10个双链RNA基因组片段，这是禽正呼肠孤病毒的典型特征。对基因组片段进行克隆、测序和序列分析后发现，与近期中国水禽呼肠孤病毒（如DRV 091）相比，HN5d的σC蛋白存在18个氨基酸的独特缺失。对编码外衣壳蛋白的片段的cDNA扩增子进行系统发育分析表明，HN5d是一种新型的2型水禽呼肠孤病毒分离株。用HN5d接种北京鸭雏鸭会导致脾脏坏死，这是2006年在中国发现的“鸭脾脏坏死病”（DSND）的典型特征。与典型的DSND不同，HN5d在实验感染鸭雏鸭的肝脏中产生更严重的出血性和/或坏死性病变。在实验暴露的鸭雏鸭中，20%-30%在最初7天内死亡。这些发现表明，HN5d是一种新型鸭呼肠孤病毒分离株，对北京鸭雏鸭具有更强的致病性。
