Chen Shao-Ying, Chen Shi-Long, Lin Feng-Qiang, Wang Shao, Jiang Bin, Cheng Xiao-Xia, Zhu Xiao-Li, Li Zhao-Long
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China.
Bing Du Xue Bao. 2012 May;28(3):224-30.
The virus strains were isolated from the liver and spleen of the dead young ducks characterized with symptoms of hemorrhagic-necrotic hepatitis. These isolates could cause the death of muscovy duck-embryo and chick-embryo. 1-day-old birds infected with these isolates had the same character with clinically dead birds and the virus could be isolated from artificially infected birds. These isolates could proliferate in MDEF and result in CPE. The virus could proliferate in the cytoplasm in order of crystals and arranged in the latlic-like. The viron was shown spherical, icosahedron, cubic symmetry, no-envelope, with double-layered capsid, about 70 nm in diameter by electron microscopy. The genome segments of the virus were consisted of L1-3, M1-3 and S1-4, which were similar to that of avian reovirus (ARV). Compared to 68.2%, 69.3% - 70.1%, respectively. The system evolution analysis showed that S3 gene coding sigmaB protein was placed in different branch of MDRV and ARV, indicating that S3 gene of the virus was different from ARV and MDRV. The main clinical symptoms and lesions of ducklings caused by the virus were different from the diseases caused by MDRV and ARV. It was concluded that the virus was a Novel duck reovirus belonging to Orthoreovirus genus of the Reoviridae family.
这些病毒株是从表现出出血性坏死性肝炎症状的死亡雏鸭的肝脏和脾脏中分离出来的。这些分离株可导致番鸭胚和鸡胚死亡。感染这些分离株的1日龄雏禽具有与临床死亡禽相同的特征，并且可以从人工感染的禽中分离到病毒。这些分离株可在鸭胚成纤维细胞（MDEF）中增殖并导致细胞病变效应（CPE）。病毒可在细胞质中呈晶体状增殖并排列成晶格状。通过电子显微镜观察，病毒粒子呈球形，二十面体，立方对称，无包膜，具有双层衣壳，直径约70nm。该病毒的基因组片段由L1 - 3、M1 - 3和S1 - 4组成，与禽呼肠孤病毒（ARV）相似。相比之下，分别为68.2%、69.3% - 70.1%。系统进化分析表明，编码σB蛋白的S3基因位于番鸭呼肠孤病毒（MDRV）和ARV的不同分支，表明该病毒的S3基因与ARV和MDRV不同。该病毒引起的雏鸭主要临床症状和病变与MDRV和ARV引起的疾病不同。结论是该病毒是一种新型鸭呼肠孤病毒，属于呼肠孤病毒科正呼肠孤病毒属。
