Wrotek Sylwia, Jędrzejewski Tomasz, Nowakowska Anna, Kozak Wiesław
Department of Immunology, Nicolaus Copernicus University, Lwowska 1, 87-100, Torun, Poland.
Department of Animal Physiology, Nicolaus Copernicus University, Lwowska 1, 87-100, Torun, Poland.
Biogerontology. 2016 Aug;17(4):715-23. doi: 10.1007/s10522-016-9636-x. Epub 2016 Feb 1.
Behavioral symptoms of sickness, such as fever and motor activity are a coordinated set of changes that develop during infection. The aim of study was to compare the sickness behaviour (SB) in healthy old and young rats treated with pyrogenic dose of endotoxin and to check their glutathione level. Before experimentation male Wistar rats were selected according to standard body mass, motor activity, and white blood cells count. Intraperitoneal injection of lipopolysaccharide (LPS) from E. coli was used to provoke SB. The level of liver glutathione, interleukin (IL) -6, deep body temperature (Tb) and motor activity were measured. Glutathione level in old and young rats did not differ significantly. In both young and old rats LPS administration provoked fever (the mean value of Tb was 38.06 ± 0.01 °C in old rats, and 38.19 ± 0.06 °C in young rats). LPS injection affected night-time activity in both groups (12 h averages were 1.56 ± 0.40 counts in old LPS-treated rats vs 2.74 ± 0.53 counts in not-treated old rats and 3.44 ± 0.60 counts for young LPS-treated vs 4.28 ± 0.57 counts for young not-treated rats). The injection of LPS provoked an elevation of plasma IL-6 concentration (from values below the lowest detectable standard in not-treated groups of animals to 6322.82 ± 537.00 pg/mL in old LPS-treated rats and 7415.62 ± 451.88 pg/mL in young LPS-treated rats). Based on these data, we conclude that good health of aged rats prevents decrease in the glutathione level. Old rats are still able to develop SB in response to pyrogenic dose of LPS, although its components have changed pattern compared to young animals.
疾病的行为症状，如发热和运动活动，是感染期间出现的一组协调变化。本研究的目的是比较用致热剂量内毒素处理的健康老年和年轻大鼠的疾病行为（SB），并检测它们的谷胱甘肽水平。实验前，根据标准体重、运动活动和白细胞计数选择雄性Wistar大鼠。腹腔注射大肠杆菌的脂多糖（LPS）以诱发SB。测量肝脏谷胱甘肽水平、白细胞介素（IL）-6、深部体温（Tb）和运动活动。老年和年轻大鼠的谷胱甘肽水平无显著差异。在年轻和老年大鼠中，LPS给药均引起发热（老年大鼠Tb的平均值为38.06±0.01°C，年轻大鼠为38.19±0.06°C）。LPS注射影响两组的夜间活动（老年LPS处理大鼠12小时平均值为1.56±0.40次计数，未处理老年大鼠为2.74±0.53次计数，年轻LPS处理大鼠为3.44±0.60次计数，年轻未处理大鼠为4.28±0.57次计数）。LPS注射导致血浆IL-6浓度升高（从未处理动物组低于最低可检测标准的值升至老年LPS处理大鼠的6322.82±537.00 pg/mL和年轻LPS处理大鼠的7415.62±451.88 pg/mL）。基于这些数据，我们得出结论，老年大鼠的良好健康状况可防止谷胱甘肽水平降低。老年大鼠仍能对致热剂量的LPS产生SB反应，尽管与年轻动物相比，其组成部分的模式有所改变。
