Wang Dan-dan, Zhu Hua-zhang, Li Shi-wei, Yang Jia-ming, Xiao Yang, Kang Qiang-rong, Li Chen-yang, Zhao Yun-shi, Zeng Yong, Li Yan, Zhang Jian, He Zhen-dan, Ying Ying
The First Affiliated Hospital of Kunming Medical University , Kunming 650000, China.
J Agric Food Chem. 2016 Feb 24;64(7):1528-39. doi: 10.1021/acs.jafc.5b05864. Epub 2016 Feb 11.
Increased apoptosis of retinal ganglion cells (RGCs) contributes to the gradual loss of retinal neurons at the early phase of diabetic retinopathy (DR). There is an urgent need to search for drugs with neuroprotective effects against apoptosis of RGCs for the early treatment of DR. This study aimed to investigate the neuroprotective effects of saponins extracted from Panax notoginseng, a traditional Chinese medicine, on apoptosis of RGCs stimulated by palmitate, a metabolic factor for the development of diabetes and its complications, and to explore the potential molecular mechanism. We showed that crude saponins of P. notoginseng (CSPN) inhibited the increased apoptosis and loss of postsynaptic protein PSD-95 by palmitate in staurosporine-differentiated RGC-5 cells. Moreover, CSPN suppressed palmitate-induced reactive oxygen species generation and endoplasmic reticulum stress-associated eIF2α/ATF4/CHOP and caspase 12 pathways. Thus, our findings address the potential therapeutic significance of CSPN for the early stage of DR.
视网膜神经节细胞（RGCs）凋亡增加是糖尿病视网膜病变（DR）早期视网膜神经元逐渐丧失的原因。迫切需要寻找对RGCs凋亡具有神经保护作用的药物用于DR的早期治疗。本研究旨在探讨从中药三七中提取的皂苷对由棕榈酸酯（一种糖尿病及其并发症发生发展的代谢因子）刺激的RGCs凋亡的神经保护作用，并探索其潜在分子机制。我们发现三七粗皂苷（CSPN）可抑制棕榈酸酯诱导的星形孢菌素分化的RGC-5细胞中凋亡增加以及突触后蛋白PSD-95的丢失。此外，CSPN可抑制棕榈酸酯诱导的活性氧生成以及内质网应激相关的eIF2α/ATF4/CHOP和半胱天冬酶12信号通路。因此，我们的研究结果揭示了CSPN在DR早期阶段的潜在治疗意义。
