Quérard Jérôme, Le Saux Thomas, Gautier Arnaud, Alcor Damien, Croquette Vincent, Lemarchand Annie, Gosse Charlie, Jullien Ludovic
Ecole Normale Supérieure-PSL Research University, Département de Chimie, 24, rue Lhomond, F-75005, Paris, France.
Sorbonne Universités, UPMC Univ Paris 06, PASTEUR, F-75005, Paris, France.
Chemphyschem. 2016 May 18;17(10):1396-413. doi: 10.1002/cphc.201500987. Epub 2016 Feb 2.
Living cells are chemical mixtures of exceptional interest and significance, whose investigation requires the development of powerful analytical tools fulfilling the demanding constraints resulting from their singular features. In particular, multiplexed observation of a large number of molecular targets with high spatiotemporal resolution appears highly desirable. One attractive road to address this analytical challenge relies on engaging the targets in reactions and exploiting the rich kinetic signature of the resulting reactive module, which originates from its topology and its rate constants. This review explores the various facets of this promising strategy. We first emphasize the singularity of the content of a living cell as a chemical mixture and suggest that its multiplexed observation is significant and timely. Then, we show that exploiting the kinetics of analytical processes is relevant to selectively detect a given analyte: upon perturbing the system, the kinetic window associated to response read-out has to be matched with that of the targeted reactive module. Eventually, we introduce the state-of-the-art of cell imaging exploiting protocols based on reaction kinetics and draw some promising perspectives.
活细胞是具有特殊意义和重要性的化学混合物，对其进行研究需要开发强大的分析工具，以满足因其独特特征而产生的严格限制。特别是，以高时空分辨率对大量分子靶点进行多重观察显得非常必要。应对这一分析挑战的一条有吸引力的途径是让靶点参与反应，并利用由此产生的反应模块丰富的动力学特征，该特征源于其拓扑结构和速率常数。本综述探讨了这一有前景的策略的各个方面。我们首先强调活细胞内容物作为化学混合物的独特性，并表明对其进行多重观察具有重要意义且恰逢其时。然后，我们表明利用分析过程的动力学与选择性检测给定分析物相关：在扰动系统时，与响应读出相关的动力学窗口必须与目标反应模块的动力学窗口相匹配。最后，我们介绍了基于反应动力学的细胞成像利用方案的最新进展，并展望了一些有前景的方向。
