PharmaCadence Analytical Services, LLC, Hatfield, PA, USA.
Immunology and Respiratory Disease, Boehringer-Ingelheim Pharmaceuticals Inc., Ridgefield, CT, USA.
Methods Mol Biol. 2025;2855:341-354. doi: 10.1007/978-1-0716-4116-3_20.
Bioactive lipid mediators derived from arachidonic acid constitute an attractive pool of metabolites that reflect cellular function and signaling, as well as potential biomarkers that may respond quantitatively to disease progression or pharmacological treatment. Their quantitative measurement in biological samples is complicated by the number of isomers that share common structural features, which are not easily distinguished by immunoassays or reverse phase chromatography-tandem mass spectrometry. Here, we present a method that enables the rapid analysis of a panel of over 25 biologically important eicosanoids in a 96-well format for cell culture supernatants, plasma, and organ tissues using convergence chromatography-tandem mass spectrometry to resolve these analytes of interest.
源自花生四烯酸的生物活性脂质介质构成了一个有吸引力的代谢物库，反映了细胞功能和信号转导，以及可能的生物标志物，这些标志物可能对疾病进展或药物治疗有定量反应。在生物样本中对它们进行定量测量很复杂，因为有许多异构体具有共同的结构特征，免疫测定或反相色谱-串联质谱技术不容易将它们区分开来。在这里，我们介绍了一种方法，该方法使用收敛色谱-串联质谱技术在 96 孔格式中快速分析细胞培养上清液、血浆和器官组织中超过 25 种具有重要生物学意义的类二十烷酸，以解决这些感兴趣的分析物。
