Larsson L, Sonesson A, Jimenez J
Medical Microbiology, Lund University, Sweden.
Eur J Clin Microbiol. 1987 Dec;6(6):729-31. doi: 10.1007/BF02013086.
Pentafluorobenzyl and pentafluoropropionyl/pentafluorobenzyl esters of bacterial fatty acids were analysed by capillary gas chromatography using both flame ionization and electron capture detection. No differences between the relative peak areas of the various fatty acids were observed as regards the two detectors used except that response of the electron capture dectector to the hydroxy acid derivatives exceeded that to the non-hydroxy acid derivatives by 20%. Use of the described derivatives in combination with electron capture detection yields chromatograms comparable with those obtained by analysis of methyl esters using flame ionization detection but with superior sensitivity.
采用火焰离子化检测和电子捕获检测，通过毛细管气相色谱法分析了细菌脂肪酸的五氟苄酯和五氟丙酰基/五氟苄酯。除电子捕获检测器对羟基酸衍生物的响应比对非羟基酸衍生物的响应高20%外，就所使用的两种检测器而言，未观察到各种脂肪酸相对峰面积之间的差异。将所述衍生物与电子捕获检测结合使用，可得到与使用火焰离子化检测分析甲酯所获得的色谱图相当但灵敏度更高的色谱图。
