Nan Ze-Dong, Zhao Ming-Bo, Zeng Ke-Wu, Tian Shuai-Hua, Wang Wei-Nan, Jiang Yong, Tu Peng-Fei
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China; Leshan Vocational & Technical college, Leshan 614000, China.
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
Chin J Nat Med. 2016 Jan;14(1):61-5. doi: 10.3724/SP.J.1009.2016.00065.
In order to determine the chemical constituents of Cistanche deserticola cultured in Tarim desert, a systematically phytochemical investigation was carried out. The constituents were isolated by silica gel, Sephadex LH-20, MCI gel, ODS column chromatography, and semi-preparative HPLC. Their structures were determined on the basis of MS and NMR spectroscopic analyses, by chemical methods, and/or comparison with literature data. The anti-inflammatory activities of the isolates were evaluated for their inhibitory effects on the lipopolysaccharide (LPS)-induced nitric oxide (NO) production in BV-2 mouse microglial cells. Nine iridoids were isolated and identified as cistadesertoside A (1), cistanin (2), cistachlorin (3), 6-deoxycatalpol (4), gluroside (5), kankanoside A (6), ajugol (7), bartsioside (8), and 8-epi-loganic acid (9). Compound 9 exhibited potent inhibition on the NO production with an IC50 value being 5.2 μmol·L(-1), comparable to the positive control quercetin (4.3 μmol·L(-1)). Compound 1 was a new iridoid, and compounds 5, 6, and 8 were isolated from this species for the first time.
为了确定塔里木沙漠种植的管花肉苁蓉的化学成分，开展了系统的植物化学研究。通过硅胶柱色谱、葡聚糖凝胶LH - 20柱色谱、MCI凝胶柱色谱、十八烷基硅烷键合硅胶（ODS）柱色谱和半制备高效液相色谱法分离其成分。根据质谱和核磁共振光谱分析、化学方法以及/或者与文献数据比较确定它们的结构。评估分离物的抗炎活性，检测其对脂多糖（LPS）诱导的BV - 2小鼠小胶质细胞产生一氧化氮（NO）的抑制作用。分离并鉴定出9种环烯醚萜类化合物，分别为管花肉苁蓉苷A（1）、管花苷（2）、管花氯苷（3）、6 - 脱氧梓醇（4）、格罗苷（5）、管花肉苁蓉新苷A（6）、筋骨草苷（7）、肉苁蓉苷（8）和8 - 表 - 裂环马钱子苷（9）。化合物9对NO的产生表现出强效抑制作用，半数抑制浓度（IC50）值为5.2 μmol·L⁻¹，与阳性对照槲皮素（4.3 μmol·L⁻¹）相当。化合物1是一种新的环烯醚萜类化合物，化合物5、6和8首次从该物种中分离得到。
