载利福平固体脂质纳米粒对表皮葡萄球菌生物膜的抗菌效果
Antibacterial efficacy of rifampin loaded solid lipid nanoparticles against Staphylococcus epidermidis biofilm.
作者信息
Fazly Bazzaz Bibi Sedigheh, Khameneh Bahman, Zarei Hamed, Golmohammadzadeh Shiva
机构信息
Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Microb Pathog. 2016 Apr;93:137-44. doi: 10.1016/j.micpath.2015.11.031. Epub 2016 Feb 4.
OBJECTIVE
The aim of the present study was to assess the in vitro anti-biofilm activities of solid lipid nanoparticles (SLN) loaded with rifampin against biofilm-producing Staphylococcus epidermidis.
METHODS
SLN were prepared and characterized for size, zeta potentials and encapsulation efficacy. The morphological and thermal properties of formulation were evaluated by TEM imagining and DSC analysis. The anti-biofilm activity of different formulations was assessed at different incubation times and concentrations by crystal violet (CV) and viable biofilm count methods.
RESULTS
The zeta potentials, particle sizes and encapsulation efficiencies of final formulations were 17 ± 0.7 mV, 101 ± 4.7 nm and approximately 70%; respectively. Rifampin-SLN was able to reduce the biomass of biofilm at time- and concentration-dependent manner. According to biofilm count results, the Rifampin-SLN was more effective for removal of the bacteria with respect to free rifampin.
CONCLUSION
The results of this study highlight the advantages and efficiency of Rifampin-SLN in biofilm eradication.
目的
本研究旨在评估负载利福平的固体脂质纳米粒（SLN）对产生物膜的表皮葡萄球菌的体外抗生物膜活性。
方法
制备SLN并对其尺寸、zeta电位和包封率进行表征。通过透射电子显微镜成像（TEM）和差示扫描量热法（DSC）分析评估制剂的形态和热性质。采用结晶紫（CV）和活菌生物膜计数法在不同孵育时间和浓度下评估不同制剂的抗生物膜活性。
结果
最终制剂的zeta电位、粒径和包封效率分别为17±0.7 mV、101±4.7 nm和约70%。利福平-SLN能够以时间和浓度依赖性方式降低生物膜的生物量。根据生物膜计数结果，利福平-SLN在去除细菌方面比游离利福平更有效。
结论
本研究结果突出了利福平-SLN在根除生物膜方面的优势和效率。
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