Silver nanoparticles (AgNPs), manganese dioxide nanoparticles (MnO₂NPs) and silver-doped manganese dioxide nanoparticles (Ag-doped MnO₂NPs) were synthesized by simultaneous green chemistry reduction approach. Aqueous extract from the leaves of medicinally important plant Cucurbita pepo was used as reducing and capping agents. Various characterization techniques were carried out to affirm the formation of nanoparticles. HR-TEM analysis confirmed the size of nanoparticles in the range of 15-70 nm and also metal doping was confirmed through XRD and EDS analyses. FT-IR analysis confirmed that the presence of biomolecules in the aqueous leaves extract was responsible for nanoparticles synthesis. Further, the concentration of metals and their doping in the reaction mixture was achieved by ICP-MS. The growth curve and well diffusion study of synthesized nanoparticles were performed against food- and water-borne Gram-positive and Gram-negative bacterial pathogens. The mode of interaction of nanoparticles on bacterial cells was demonstrated through Bio-TEM analysis. Interestingly, AgNPs and Ag-doped MnO₂NPs showed better antibacterial activity against all the tested bacterial pathogens; however, MnO₂NPs alone did not show any antibacterial properties. Hence, AgNPs and Ag-doped MnO₂NPs synthesized from aqueous plant leaves extract may have important role in controlling various food spoilage caused by bacteria.