Augustine Bibin Baby, Dash Suvakanta, Lahkar Mangala, Sarma Usha, Samudrala Pavan Kumar, Thomas Jaya Mary
Laboratory of Tumor Pharmacology, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Guwahati, Assam, India.
Department of Pharmaceutics, Girijananda Institute of Pharmaceutical Sciences, Azara, Guwahati, Assam, India.
Pharmacogn Mag. 2014 Apr;10(38):118-24. doi: 10.4103/0973-1296.131022.
North East India is a rich source of medicinal plants and a number of plant extracts are used by tribal peoples living in this area for various disorders. L.aspera is such a plant, traditionally used as an antitumor agent.
In the present study, aerial parts of L.aspera were investigated for antitumor activity in Dalton's lymphoma (DAL) bearing mice. The ability of plant extract in free radical scavenging, neoangiogenesis inhibition and macrophage stimulation were also checked.
Based on the preliminary in vitro cytotoxicity studies ethyl acetate fraction of L.aspera (EALA) was selected for the detailed study. DAL ascites tumor model was performed to check the antitumor activity of EALA (200 and 400mg/kg of body weight). Hematological and histopathological parameters were estimated. Antioxidant levels, neoangiogenesis and peritoneal macrophage count were also determined.
In vitro MTT and Trypan blue assay results showed the cytotoxic effect of EALA in DAL cells lines. EALA treatment resulted in significant decrease in ascites tumor volume and viable cell count. Hematological and liver antioxidant parameters were normalised by EALA treatment. It was also found that EALA treatment inhibits neovascularisation and produce macrophage stimulation in treated mice.
The results showed that EALA is a promising anticancer agent and its activity is comparable to the standard drug 5-Flouro uracil (5-FU).
印度东北部是药用植物的丰富来源，该地区的部落居民使用多种植物提取物治疗各种疾病。糙叶榕就是这样一种植物，传统上用作抗肿瘤剂。
在本研究中，对糙叶榕地上部分在荷道尔顿淋巴瘤（DAL）小鼠中的抗肿瘤活性进行了研究。还检测了植物提取物的自由基清除能力、新血管生成抑制能力和巨噬细胞刺激能力。
基于初步的体外细胞毒性研究，选择糙叶榕乙酸乙酯部位（EALA）进行详细研究。采用DAL腹水瘤模型检测EALA（200和400mg/kg体重）的抗肿瘤活性。评估血液学和组织病理学参数。还测定了抗氧化水平、新血管生成和腹腔巨噬细胞计数。
体外MTT和台盼蓝试验结果显示EALA对DAL细胞系具有细胞毒性作用。EALA处理导致腹水瘤体积和活细胞计数显著降低。EALA处理使血液学和肝脏抗氧化参数恢复正常。还发现EALA处理可抑制新血管生成并在处理的小鼠中产生巨噬细胞刺激作用。
结果表明EALA是一种有前景的抗癌剂，其活性与标准药物5-氟尿嘧啶（5-FU）相当。
