Mossong Joël, Mughini-Gras Lapo, Penny Christian, Devaux Anthony, Olinger Christophe, Losch Serge, Cauchie Henry-Michel, van Pelt Wilfrid, Ragimbeau Catherine
National Health Laboratory (LNS), Surveillance and Epidemiology of Infectious Diseases, 1 rue Louis Rech, Dudelange L-3555, Luxembourg.
National Institute for Public Health and the Environment (RIVM), Centre for Infectious Disease Control (CIb). PO Box 1 - 3720 BA Bilthoven, The Netherlands.
Sci Rep. 2016 Feb 10;6:20939. doi: 10.1038/srep20939.
Campylobacteriosis has increased markedly in Luxembourg during recent years. We sought to determine which Campylobacter genotypes infect humans, where they may originate from, and how they may infect humans. Multilocus sequence typing was performed on 1153 Campylobacter jejuni and 136 C. coli human strains to be attributed to three putative animal reservoirs (poultry, ruminants, pigs) and to environmental water using the asymmetric island model. A nationwide case-control study (2010-2013) for domestic campylobacteriosis was also conducted, including 367 C. jejuni and 48 C. coli cases, and 624 controls. Risk factors were investigated by Campylobacter species, and for strains attributed to different sources using a combined case-control and source attribution analysis. 282 sequence types (STs) were identified: ST-21, ST-48, ST-572, ST-50 and ST-257 were prevailing. Most cases were attributed to poultry (61.2%) and ruminants (33.3%). Consuming chicken outside the home was the dominant risk factor for both Campylobacter species. Newly identified risk factors included contact with garden soil for either species, and consuming beef specifically for C. coli. Poultry-associated campylobacteriosis was linked to poultry consumption in wintertime, and ruminant-associated campylobacteriosis to tap-water provider type. Besides confirming chicken as campylobacteriosis primary source, additional evidence was found for other reservoirs and transmission routes.
近年来，卢森堡的弯曲杆菌病显著增加。我们试图确定哪些弯曲杆菌基因型感染人类、它们可能的来源以及它们如何感染人类。采用非对称岛屿模型，对1153株空肠弯曲杆菌和136株结肠弯曲杆菌的人类菌株进行多位点序列分型，以确定其可能的三个动物宿主（家禽、反刍动物、猪）和环境水体来源。还开展了一项全国性的家庭弯曲杆菌病病例对照研究（2010 - 2013年），包括367例空肠弯曲杆菌病例、48例结肠弯曲杆菌病例和624名对照。通过弯曲杆菌种类以及使用病例对照与来源归因分析相结合的方法，对归因于不同来源的菌株调查危险因素。共鉴定出282种序列类型（STs）：ST-21、ST-48、ST-572、ST-50和ST-257最为常见。大多数病例归因于家禽（61.2%）和反刍动物（33.3%）。外出食用鸡肉是两种弯曲杆菌的主要危险因素。新发现的危险因素包括两种弯曲杆菌与花园土壤的接触，以及结肠弯曲杆菌与食用牛肉有关。家禽相关的弯曲杆菌病与冬季食用家禽有关，反刍动物相关的弯曲杆菌病与自来水供应商类型有关。除了证实鸡肉是弯曲杆菌病的主要来源外，还发现了其他宿主和传播途径的额外证据。
