Department of Animal Health, University of Cordoba, Animal Health Building, Campus Universitario de Rabanales, 14014, Córdoba, Spain.
Department of Animal Production, University of Cordoba, Production Animal Building, Campus Universitario de Rabanales, 14014, Córdoba, Spain.
Vet Res Commun. 2022 Dec;46(4):1325-1330. doi: 10.1007/s11259-022-09981-w. Epub 2022 Sep 12.
The aim of this study was to determine the prevalence of the htrA, htrB and ppk1 genes -all of which are related to environmental persistence- in C. jejuni and C. coli isolates obtained from abattoir samples at the arrival of broilers (initial stage) and in meat products after processing (final stage). A total of 119 DNA extracts (55 C. jejuni and 64 C. coli) were included in the study. Identification of genes was performed by conventional PCR (one for each gene). The overall prevalence was 40.3%, 93.3% and 68.9% for the htrA, htrB and ppk1 genes, respectively. Statistically significant differences were found (p < 0.05) between prevalence of C. jejuni and C. coli for all three genes. In C. coli the prevalence was significantly higher for the htrA (p = 0.007) and htrB (p = 0.015) genes, while ppk1 gene prevalence was significantly higher in C. jejuni (p < 0.001). In addition, statistically significant increase in the frequency of htrA (p = 0.007) and htrB (p = 0.013) genes in the final product compared to broilers on arrival at the abattoir was observed in C. jejuni, but not in C. coli. These results suggest that htrA and htrB genes are involved in environmental persistence of Campylobacter jejuni.
本研究旨在确定 htrA、htrB 和 ppk1 基因的流行率 - 这些基因均与环境持久性相关 - 在从到达肉鸡屠宰场时的初始阶段的屠宰场样本和加工后的肉类产品中获得的空肠弯曲菌和大肠弯曲菌分离株中。研究共纳入了 119 份 DNA 提取物（55 份空肠弯曲菌和 64 份大肠弯曲菌）。通过常规 PCR（每种基因一个）进行基因鉴定。htrA、htrB 和 ppk1 基因的总体流行率分别为 40.3%、93.3%和 68.9%。htrA、htrB 和 ppk1 基因在空肠弯曲菌和大肠弯曲菌之间的流行率存在统计学差异（p<0.05）。在大肠弯曲菌中，htrA（p=0.007）和 htrB（p=0.015）基因的流行率显著更高，而空肠弯曲菌中 ppk1 基因的流行率显著更高（p<0.001）。此外，与到达屠宰场时的肉鸡相比，空肠弯曲菌在最终产品中 htrA（p=0.007）和 htrB（p=0.013）基因的频率显著增加，但在大肠弯曲菌中则没有。这些结果表明 htrA 和 htrB 基因参与了空肠弯曲菌的环境持久性。
