Ambatipudi Srikant, Cuenin Cyrille, Hernandez-Vargas Hector, Ghantous Akram, Le Calvez-Kelm Florence, Kaaks Rudolf, Barrdahl Myrto, Boeing Heiner, Aleksandrova Krasimira, Trichopoulou Antonia, Lagiou Pagona, Naska Androniki, Palli Domenico, Krogh Vittorio, Polidoro Silvia, Tumino Rosario, Panico Salvatore, Bueno-de-Mesquita Bas, Peeters Petra Hm, Quirós José Ramón, Navarro Carmen, Ardanaz Eva, Dorronsoro Miren, Key Tim, Vineis Paolo, Murphy Neil, Riboli Elio, Romieu Isabelle, Herceg Zdenko
International Agency for Research on Cancer (IARC), Lyon, France.
Division of Cancer Epidemiology, German Cancer Research Center (DKFZ), Heidelberg, Germany.
Epigenomics. 2016 May;8(5):599-618. doi: 10.2217/epi-2016-0001. Epub 2016 Feb 11.
Epigenetic changes may occur in response to environmental stressors, and an altered epigenome pattern may represent a stable signature of environmental exposure.
MATERIALS & METHODS: Here, we examined the potential of DNA methylation changes in 910 prediagnostic peripheral blood samples as a marker of exposure to tobacco smoke in a large multinational cohort.
We identified 748 CpG sites that were differentially methylated between smokers and nonsmokers, among which we identified novel regionally clustered CpGs associated with active smoking. Importantly, we found a marked reversibility of methylation changes after smoking cessation, although specific genes remained differentially methylated up to 22 years after cessation.
Our study has comprehensively cataloged the smoking-associated DNA methylation alterations and showed that these alterations are reversible after smoking cessation.
表观遗传变化可能因环境应激源而发生，表观基因组模式的改变可能代表环境暴露的稳定特征。
在此，我们在一个大型跨国队列中，研究了910份诊断前外周血样本中DNA甲基化变化作为接触烟草烟雾标志物的潜力。
我们鉴定出748个在吸烟者和非吸烟者之间甲基化差异的CpG位点，其中我们发现了与主动吸烟相关的新的区域聚集CpG。重要的是，我们发现戒烟后甲基化变化具有显著的可逆性，尽管特定基因在戒烟后长达22年仍存在甲基化差异。
我们的研究全面编目了与吸烟相关的DNA甲基化改变，并表明这些改变在戒烟后是可逆的。
