Peralbo-Molina A, Calderón-Santiago M, Priego-Capote F, Jurado-Gámez B, Luque de Castro M D
Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Córdoba, 14014, Córdoba, Spain. Institute of Biomedical Research Maimónides (IMIBIC), Reina Sofía Hospital, University of Córdoba, 14004 Córdoba, Spain.
J Breath Res. 2016 Feb 11;10(1):016011. doi: 10.1088/1752-7155/10/1/016011.
The search for new clinical tests aimed at diagnosing chronic respiratory diseases is a current research line motivated by the lack of efficient screening tools and the severity of some of these pathologies. Alternative biological samples can open the door to new screening tools. A promising biofluid that is rarely used for diagnostic purposes is exhaled breath condensate (EBC), the composition of which has been inadequately studied. In this research, untargeted analysis of EBC using gas chromatography time-of-flight mass spectrometry has been applied to a cohort of patients with lung cancer (n = 48), risk factor individuals (active smokers and ex-smokers, n = 130) and control healthy individuals (non-smokers without respiratory diseases, n = 61). An identical protocol was applied to the two EBC fractions provided by the sampling device (upper and central airways and distal airway) from each individual, which allowed the compositional differences between the two EBC fractions to be detected. Tentative compounds that contribute to discrimination between the three groups were identified, and a relevant role for lipids such as monoacylglycerols and squalene was found. These results could support the ability of metabolomics to go inside the study of lung cancer.
寻找旨在诊断慢性呼吸道疾病的新临床试验是当前的一条研究路线，其动机在于缺乏有效的筛查工具以及其中一些病症的严重性。替代性生物样本可为新的筛查工具打开大门。一种很少用于诊断目的但很有前景的生物流体是呼出气冷凝物（EBC），其成分尚未得到充分研究。在本研究中，使用气相色谱飞行时间质谱法对EBC进行非靶向分析，已应用于一组肺癌患者（n = 48）、有风险因素个体（现吸烟者和既往吸烟者，n = 130）以及对照健康个体（无呼吸道疾病的非吸烟者，n = 61）。对采样装置从每个个体提供的两个EBC部分（上呼吸道和中央气道以及远端气道）应用相同的方案，这使得能够检测到两个EBC部分之间的成分差异。确定了有助于区分三组的暂定化合物，并发现了单酰甘油和角鲨烯等脂质的相关作用。这些结果可为代谢组学深入研究肺癌的能力提供支持。
