Panek Michał, Jonakowski Mateusz, Zioło Jan, Pietras Tadeusz, Wieteska Łukasz, Małachowska Beata, Mokros Łukasz, Szemraj Janusz, Kuna Piotr
Department of Internal Medicine, Asthma and Allergy, Medical University of Lodz, 22 Kopcinskiego St., 90-153, Lodz, Poland.
Students Research Group at the Department of Internal Medicine, Asthma and Allergy, Medical University of Lodz, 22 Kopcinskiego St., 90-153, Lodz, Poland.
Mol Neurobiol. 2017 Apr;54(3):1733-1744. doi: 10.1007/s12035-016-9768-7. Epub 2016 Feb 13.
Asthma is a chronic inflammatory and heterogeneous disease developing mostly through allergic inflammation, which modifies the expression of various cytokines and neurotrophins. Previous studies suggest the involvement of interleukin (IL)-15 in the regulation of immune response in asthma. Brain-derived neurotrophic factor (BDNF) II plays an important role as a regulator of development and survival of neurons as well as maintenance of their physiological activity. Chronic stress associated with asthma and elevated IL-15 mRNA and BDNFII mRNA levels may affect the mood and a subjective sensation of dyspnoea-inducing anxiety. Psychopathological variables and numerous cytokine/neurotrophin interactions influence the formation of temperament and strategies of coping with stress. The aim of the study was to identify the role of IL-15 mRNA and BDNFII mRNA expressions and their effect on components of temperament and strategies of coping with stress in asthmatics. A total of 352 subjects (176 healthy volunteers and 176 asthmatic patients) participated in the study. The Formal Characteristic of Behaviour-Temperament Inventory (FCB-TI), Coping Inventory for Stressful Situations (CISS), Beck Depression Inventory, State-Trait Anxiety Inventory, and Borg Rating of Perceived Exertion (RPE) Scale were applied in all the subjects. The expression of IL-15 and BDNFII gene was measured using quantitative real-time polymerase chain reaction (qRT-PCR). Different levels of IL-15 and BDNFII expressions between healthy volunteers and patients were revealed in the study. IL-15 enhanced the BDNFII mRNA expression among patients with bronchial asthma. The depression level negatively correlated with the BDNFII mRNA expression. This neurotrophin modified the temperament variable. BDNFII significantly affected (proportional relationship) the level of briskness in asthmatic patients. BDNFII might influence the level and style of coping with stress (emotion-oriented style). This hypothesis requires further studies on protein functional models. The obtained data confirms the role of IL-15 and BDNFII in the pathomechanisms of depression and formation of selected traits defining the temperament in asthmatics.
哮喘是一种慢性炎症性异质性疾病，主要通过变应性炎症发展，这种炎症会改变多种细胞因子和神经营养因子的表达。先前的研究表明白细胞介素（IL）-15参与哮喘免疫反应的调节。脑源性神经营养因子（BDNF）II作为神经元发育和存活的调节因子以及维持其生理活性发挥着重要作用。与哮喘相关的慢性应激以及IL-15 mRNA和BDNFII mRNA水平升高可能会影响情绪和诱发呼吸困难的焦虑主观感受。心理病理学变量和众多细胞因子/神经营养因子相互作用会影响气质的形成和应对压力的策略。该研究的目的是确定IL-15 mRNA和BDNFII mRNA表达的作用及其对哮喘患者气质成分和应对压力策略的影响。共有352名受试者（176名健康志愿者和176名哮喘患者）参与了该研究。所有受试者均应用了行为气质形式特征量表（FCB-TI）、应激情境应对量表（CISS）、贝克抑郁量表、状态-特质焦虑量表和博格自觉用力程度（RPE）量表。使用定量实时聚合酶链反应（qRT-PCR）测量IL-15和BDNFII基因的表达。该研究揭示了健康志愿者和患者之间IL-15和BDNFII表达的不同水平。在支气管哮喘患者中，IL-15增强了BDNFII mRNA的表达。抑郁水平与BDNFII mRNA表达呈负相关。这种神经营养因子改变了气质变量。BDNFII显著影响（比例关系）哮喘患者的轻快程度水平。BDNFII可能会影响应对压力的水平和方式（情绪导向型方式）。这一假设需要进一步对蛋白质功能模型进行研究。所获得的数据证实了IL-15和BDNFII在哮喘患者抑郁症发病机制以及定义气质的某些特征形成中的作用。
