活的和热灭活的益生菌：对葡聚糖硫酸钠（DSS）诱导的大鼠慢性实验性结肠炎的影响。
Live and heat-killed probiotic: effects on chronic experimental colitis induced by dextran sulfate sodium (DSS) in rats.
作者信息
Sang Li-Xuan, Chang Bing, Wang Bing-Yuan, Liu Wei-Xin, Jiang Min
机构信息
Department of Geriatrics, First Affiliated Hospital of China Medical University Shenyang 110001, Liaoning, China.
Department of Gastroenterology, First Affiliated Hospital of China Medical University Shenyang 110001, Liaoning, China.
出版信息
Int J Clin Exp Med. 2015 Nov 15;8(11):20072-8. eCollection 2015.
Although a series of studies have shown that VSL#3 (L plantarum, L Bulgaricus, L casei and L. acidophilus, B breve, B longum and B infantis and S salivarius subspecies thermophilus) can exert therapeutic effects on colitis, whether heat-killed VSL#3 also can exert similar effects has never been tested. The aim of the study was to investigate whether heat-killed VSL#3 exert therapeutic effects in chronic experimental colitis by inhibiting STAT3 pathway. Chronic experimental colitis was induced by dextran sulfate sodium (DSS) in rats. Rats underwent gavage once daily for seven days with heat-killed VSL#3 (0.6 g/kg/day). The disease activity index (DAI), histological score, colon length and myeloperoxidase (MPO) activity was observed. Expression of inflammatory related mediators (STAT3, P-STAT3) and cytokines (IL-6, IL-23) in colonic tissue were detected. The results showed that live and heat-killed VSL#3 have identical anti-inflammatory effects by the assessed DAI, colon length, histological score and MPO activity. Live and heat-killed VSL#3 results in reduced IL-6, IL-23, STAT3 and P-STAT3 expression in colonic tissue. Heat-killed VSL#3 have showed significant anti-inflammatory effects by suppressing STAT3 pathway.
尽管一系列研究表明，VSL#3（植物乳杆菌、保加利亚乳杆菌、干酪乳杆菌、嗜酸乳杆菌、短双歧杆菌、长双歧杆菌、婴儿双歧杆菌和嗜热栖热唾液链球菌亚种）对结肠炎具有治疗作用，但热灭活的VSL#3是否也能发挥类似作用尚未得到验证。本研究的目的是通过抑制STAT3通路，探讨热灭活的VSL#3在慢性实验性结肠炎中是否具有治疗作用。采用葡聚糖硫酸钠（DSS）诱导大鼠慢性实验性结肠炎。大鼠每天灌胃一次热灭活的VSL#3（0.6 g/kg/天），连续7天。观察疾病活动指数（DAI）、组织学评分、结肠长度和髓过氧化物酶（MPO）活性。检测结肠组织中炎症相关介质（STAT3、P-STAT3）和细胞因子（IL-6、IL-23）的表达。结果显示，通过评估DAI、结肠长度、组织学评分和MPO活性，活的和热灭活的VSL#3具有相同的抗炎作用。活的和热灭活的VSL#3均可降低结肠组织中IL-6、IL-23、STAT3和P-STAT3的表达。热灭活的VSL#3通过抑制STAT3通路显示出显著的抗炎作用。
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