School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
J Clin Microbiol. 2010 Nov;48(11):4279-82. doi: 10.1128/JCM.01360-10. Epub 2010 Sep 22.
Mucosa-associated bacterial flora from control individuals and inflammatory bowel disease (IBD) patients were evaluated by real-time analysis using 16S rRNA-based genus-specific primers. Our data show a clear delineation in concentration of bacteria between the predominating and subdominating genera under disease conditions, indicating that the subsets of bacteria participating in the pathogenesis of ulcerative colitis (UC) and Crohn's disease (CD) are likely to be different.
采用基于 16S rRNA 的属特异性引物的实时分析方法评估了对照个体和炎症性肠病(IBD)患者的黏膜相关细菌菌群。我们的数据显示,在疾病状态下,主要属和次要属之间的细菌浓度有明显的差异,表明参与溃疡性结肠炎(UC)和克罗恩病(CD)发病机制的细菌亚群可能不同。