• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

酵母HSP82基因的基础水平表达需要一个热休克调节元件。

Basal-level expression of the yeast HSP82 gene requires a heat shock regulatory element.

作者信息

McDaniel D, Caplan A J, Lee M S, Adams C C, Fishel B R, Gross D S, Garrard W T

机构信息

Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235.

出版信息

Mol Cell Biol. 1989 Nov;9(11):4789-98. doi: 10.1128/mcb.9.11.4789-4798.1989.

DOI:10.1128/mcb.9.11.4789-4798.1989
PMID:2689867
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363627/
Abstract

Previous studies have shown that heat shock factor is constitutively bound to heat shock elements in Saccharomyces cerevisiae. We demonstrate that mutation of the heat shock element closest to the TATA box of the yeast HSP82 promoter abolishes basal-level transcription without markedly affecting inducibility. The mutated heat shock element no longer bound putative heat shock factor, either in vitro or in vivo, but still resided within a nuclease-hypersensitive site in the chromatin. Thus, constitutive binding of heat shock factor to heat shock elements in S. cerevisiae appears to functionally direct basal-level transcription.

摘要

先前的研究表明，热休克因子在酿酒酵母中与热休克元件组成性结合。我们证明，酵母HSP82启动子中最靠近TATA盒的热休克元件发生突变会消除基础水平的转录，而不会显著影响诱导性。无论是在体外还是体内，突变的热休克元件都不再与假定的热休克因子结合，但仍位于染色质中的核酸酶超敏位点内。因此，热休克因子与酿酒酵母中热休克元件的组成性结合似乎在功能上指导基础水平的转录。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6a/363627/2795ea409b93/molcellb00059-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6a/363627/becbb099f3b6/molcellb00059-0216-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6a/363627/b8a716c17cf9/molcellb00059-0217-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6a/363627/0be66de6b272/molcellb00059-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6a/363627/8a44494e99bd/molcellb00059-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6a/363627/f4483c7dd06e/molcellb00059-0219-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6a/363627/2795ea409b93/molcellb00059-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6a/363627/becbb099f3b6/molcellb00059-0216-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6a/363627/b8a716c17cf9/molcellb00059-0217-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6a/363627/0be66de6b272/molcellb00059-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6a/363627/8a44494e99bd/molcellb00059-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6a/363627/f4483c7dd06e/molcellb00059-0219-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6a/363627/2795ea409b93/molcellb00059-0220-a.jpg

相似文献

1
Basal-level expression of the yeast HSP82 gene requires a heat shock regulatory element.酵母HSP82基因的基础水平表达需要一个热休克调节元件。
Mol Cell Biol. 1989 Nov;9(11):4789-98. doi: 10.1128/mcb.9.11.4789-4798.1989.
2
Genomic footprinting of the yeast HSP82 promoter reveals marked distortion of the DNA helix and constitutive occupancy of heat shock and TATA elements.酵母HSP82启动子的基因组足迹分析揭示了DNA螺旋的显著扭曲以及热休克元件和TATA元件的组成型占据。
J Mol Biol. 1990 Dec 5;216(3):611-31. doi: 10.1016/0022-2836(90)90387-2.
3
Multiple protein-DNA interactions over the yeast HSC82 heat shock gene promoter.酵母HSC82热休克基因启动子上的多种蛋白质 - DNA相互作用。
Nucleic Acids Res. 1995 May 25;23(10):1822-9. doi: 10.1093/nar/23.10.1822.
4
Heat shock factor gains access to the yeast HSC82 promoter independently of other sequence-specific factors and antagonizes nucleosomal repression of basal and induced transcription.热休克因子可独立于其他序列特异性因子进入酵母HSC82启动子，并拮抗基础转录和诱导转录的核小体抑制作用。
Mol Cell Biol. 1996 Dec;16(12):7004-17. doi: 10.1128/MCB.16.12.7004.
5
Promoter function and in situ protein/DNA interactions upstream of the yeast HSP90 heat shock genes.酵母HSP90热休克基因上游的启动子功能及原位蛋白质/DNA相互作用
Antonie Van Leeuwenhoek. 1990 Oct;58(3):175-86. doi: 10.1007/BF00548930.
6
Uncoupling gene activity from chromatin structure: promoter mutations can inactivate transcription of the yeast HSP82 gene without eliminating nucleosome-free regions.使基因活性与染色质结构解偶联：启动子突变可使酵母HSP82基因的转录失活，而不消除无核小体区域。
Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9166-70. doi: 10.1073/pnas.89.19.9166.
7
Conditional silencing: the HMRE mating-type silencer exerts a rapidly reversible position effect on the yeast HSP82 heat shock gene.条件性沉默：HMRE交配型沉默子对酵母HSP82热休克基因施加快速可逆的位置效应。
Mol Cell Biol. 1993 Feb;13(2):727-38. doi: 10.1128/mcb.13.2.727-738.1993.
8
A critical role for heat shock transcription factor in establishing a nucleosome-free region over the TATA-initiation site of the yeast HSP82 heat shock gene.热休克转录因子在酵母HSP82热休克基因的TATA起始位点上建立无核小体区域中起关键作用。
EMBO J. 1993 Oct;12(10):3931-45. doi: 10.1002/j.1460-2075.1993.tb06071.x.
9
A bipartite operator interacts with a heat shock element to mediate early meiotic induction of Saccharomyces cerevisiae HSP82.一种二分体算子与热休克元件相互作用，以介导酿酒酵母HSP82的早期减数分裂诱导。
Mol Cell Biol. 1995 Dec;15(12):6754-69. doi: 10.1128/MCB.15.12.6754.
10
The promoter region of the yeast KAR2 (BiP) gene contains a regulatory domain that responds to the presence of unfolded proteins in the endoplasmic reticulum.酵母KAR2（BiP）基因的启动子区域包含一个调控结构域，该结构域可对内质网中未折叠蛋白的存在做出反应。
Mol Cell Biol. 1993 Feb;13(2):877-90. doi: 10.1128/mcb.13.2.877-890.1993.

引用本文的文献

1
Hsf1 is essential for proteotoxic stress response in smyd1b-deficient embryos and fish survival under heat shock.Hsf1对于smyd1b缺陷型胚胎中的蛋白毒性应激反应以及热休克条件下鱼类的存活至关重要。
FASEB J. 2025 Jan 15;39(1):e70283. doi: 10.1096/fj.202401875R.
2
Genetic inactivation of essential reveals an isolated transcriptional stress response selectively induced by protein misfolding.必需基因的遗传失活揭示了一种选择性诱导的孤立转录应激反应，该反应由蛋白质错误折叠引起。
Mol Biol Cell. 2023 Sep 1;34(10):ar101. doi: 10.1091/mbc.E23-05-0153. Epub 2023 Jul 19.
3
Hsf1 and Hsp70 constitute a two-component feedback loop that regulates the yeast heat shock response.

本文引用的文献

1
The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis.使用增感屏或有机闪烁体来可视化通过凝胶电泳分离的放射性分子。
Methods Enzymol. 1980;65(1):363-71. doi: 10.1016/s0076-6879(80)65047-2.
2
The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.染色质中果蝇热休克基因的5'端对脱氧核糖核酸酶I高度敏感。
Nature. 1980 Aug 28;286(5776):854-60. doi: 10.1038/286854a0.
3
A Drosophila RNA polymerase II transcription factor contains a promoter-region-specific DNA-binding activity.
Hsf1 和 Hsp70 构成了一个双组分反馈回路，调节酵母热休克反应。
Elife. 2018 Feb 2;7:e31668. doi: 10.7554/eLife.31668.
4
Evidence for Multiple Mediator Complexes in Yeast Independently Recruited by Activated Heat Shock Factor.酵母中多个中介复合物被激活的热休克因子独立招募的证据。
Mol Cell Biol. 2016 Jun 29;36(14):1943-60. doi: 10.1128/MCB.00005-16. Print 2016 Jul 15.
5
Expression of three topologically distinct membrane proteins elicits unique stress response pathways in the yeast Saccharomyces cerevisiae.三种拓扑结构不同的膜蛋白的表达在酿酒酵母中引发独特的应激反应途径。
Physiol Genomics. 2015 Jun;47(6):198-214. doi: 10.1152/physiolgenomics.00101.2014. Epub 2015 Mar 10.
6
Deteriorated stress response in stationary-phase yeast: Sir2 and Yap1 are essential for Hsf1 activation by heat shock and oxidative stress, respectively.稳定期酵母中应激反应的恶化：Sir2和Yap1分别对于热休克和氧化应激激活Hsf1至关重要。
PLoS One. 2014 Oct 30;9(10):e111505. doi: 10.1371/journal.pone.0111505. eCollection 2014.
7
Fungal Hsp90: a biological transistor that tunes cellular outputs to thermal inputs.真菌 Hsp90：一种生物晶体管，可调节细胞输出以适应热输入。
Nat Rev Microbiol. 2012 Oct;10(10):693-704. doi: 10.1038/nrmicro2875.
8
The response to heat shock and oxidative stress in Saccharomyces cerevisiae.酵母细胞应对热休克和氧化应激的反应。
Genetics. 2012 Apr;190(4):1157-95. doi: 10.1534/genetics.111.128033. Epub 2011 Dec 29.
9
SAGA and Rpd3 chromatin modification complexes dynamically regulate heat shock gene structure and expression.SAGA和Rpd3染色质修饰复合物动态调节热休克基因的结构和表达。
J Biol Chem. 2009 Nov 20;284(47):32914-31. doi: 10.1074/jbc.M109.058610. Epub 2009 Sep 15.
10
A functional module of yeast mediator that governs the dynamic range of heat-shock gene expression.酵母中介体的一个功能模块，其调控热休克基因表达的动态范围。
Genetics. 2006 Apr;172(4):2169-84. doi: 10.1534/genetics.105.052738. Epub 2006 Feb 1.
一种果蝇RNA聚合酶II转录因子含有启动子区域特异性DNA结合活性。
Cell. 1984 Feb;36(2):357-69. doi: 10.1016/0092-8674(84)90229-0.
4
Heat shock-regulated production of Escherichia coli beta-galactosidase in Saccharomyces cerevisiae.热休克调节酿酒酵母中大肠杆菌β-半乳糖苷酶的产生。
Mol Cell Biol. 1983 Sep;3(9):1625-33. doi: 10.1128/mcb.3.9.1625-1633.1983.
5
Changing patterns of gene expression during sporulation in yeast.酵母孢子形成过程中基因表达模式的变化。
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7323-7. doi: 10.1073/pnas.81.23.7323.
6
Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
J Bacteriol. 1983 Jan;153(1):163-8. doi: 10.1128/jb.153.1.163-168.1983.
7
Genomic sequencing.基因组测序
Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5. doi: 10.1073/pnas.81.7.1991.
8
Two protein-binding sites in chromatin implicated in the activation of heat-shock genes.染色质中与热休克基因激活有关的两个蛋白质结合位点。
Nature. 1984;309(5965):229-34. doi: 10.1038/309229a0.
9
Complete sequence of the heat shock-inducible HSP90 gene of Saccharomyces cerevisiae.酿酒酵母热激诱导型HSP90基因的完整序列
J Biol Chem. 1984 May 10;259(9):5745-51.
10
Identification and expression of a cloned yeast heat shock gene.一个克隆的酵母热休克基因的鉴定与表达。
J Biol Chem. 1983 Feb 10;258(3):1908-13.