Zheng Yingying, Miao Jianjun, Zhang Fuming, Cai Chao, Koh Amanda, Simmons Trevor J, Mousa Shaker A, Linhardt Robert J
Department of Physics and Key Laboratory of ATMMT Ministry of Education, Zhejiang Sci-Tech University, Hangzhou, 310018, People's Republic of China.
Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, New York, 12180-3590, USA.
React Funct Polym. 2016 Mar 1;100:142-150. doi: 10.1016/j.reactfunctpolym.2016.01.013.
A functional anticoagulant and anti-bacterial coating for polyethylene (PE) films is described. The stepwise preparation of this nanocomposite surface coating involves O plasma etching of PE film, carbodiimide coupling of cysteamine to the etched PE film, binding of Ag to sulfhydryl groups of cysteamine, and assembly of heparin capped AgNPs on the PE film. The nanocomposite film and its components were characterized by H-nuclear magnetic resonance spectroscopy, attenuated total reflectance-Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and field emission-scanning electron microscopy. The resulting PE films demonstrate anticoagulant activity using a hemoglobin whole blood clotting assay, and anti-bacterial activity against 3551 (Gram-positive) and BL21 (Gram-negative) bacteria. The hydrophilicity of the heparin coated PE was determined by contact angle measurements; and the stability of the nanocomposite film, with respect to Ag leaching, was assessed by atomic absorption spectroscopy.
本文描述了一种用于聚乙烯（PE）薄膜的功能性抗凝抗菌涂层。这种纳米复合表面涂层的逐步制备过程包括对PE薄膜进行O等离子体蚀刻、将半胱胺通过碳二亚胺偶联到蚀刻后的PE薄膜上、使Ag与半胱胺的巯基结合，以及在PE薄膜上组装肝素封端的AgNPs。通过氢核磁共振光谱、衰减全反射傅里叶变换红外光谱、X射线光电子能谱和场发射扫描电子显微镜对纳米复合薄膜及其组分进行了表征。所得的PE薄膜在血红蛋白全血凝血试验中表现出抗凝活性，并且对3551（革兰氏阳性）和BL21（革兰氏阴性）细菌具有抗菌活性。通过接触角测量确定了肝素涂层PE的亲水性；通过原子吸收光谱评估了纳米复合薄膜相对于Ag浸出的稳定性。
