Identification and expression analysis under abiotic stress of the - genes in L.

The R2R3-MYB gene family is the largest MYB subfamily in plants and is involved in the regulation of plant secondary metabolism and specific morphogenesis, as well as the response to biotic and abiotic stress. However, a systematic identification and characterization of this gene family has not been carried out in . In this study, we performed a transcriptome-wide survey from four tissues of to determine the genetic variation and expression pattern of the - genes. We analyzed 45 and identified 42 with a complete coding sequence via conserved motif searches. The MYB domain and other motifs in are highly conserved with AtMYBs. Phylogenetic analysis of the GbMYBs and AtMYBs categorized the R2R3-MYBs into 26 subgroups, of which 11 subgroups included proteins from both and , and 1 subgroup was specific to . Moreover, the expression patterns were analyzed in different tissues and abiotic stress conditions. The results revealed that were differentially expressed in various tissues and following abiotic stresses and phytohormone treatments, indicating their possible roles in biological processes and abiotic stress tolerance and adaptation. Our study demonstrated the functional diversity of the and will provide a foundation for future research into their biological and molecular functions.