Naghdi Majid, Maghbool Maryam, Seifalah-Zade Morteza, Mahaldashtian Maryam, Makoolati Zohreh, Kouhpayeh Seyed Amin, Ghasemi Afsaneh, Fereydouni Narges
Department of Anatomical Sciences, Medicine School, Fasa University of Medical Sciences, Fasa 7461686688, Iran.
Department of Pathology, Faculty of Medicine, Fasa University of Medical Sciences, Fasa 7461686688, Iran.
Evid Based Complement Alternat Med. 2016;2016:2539127. doi: 10.1155/2016/2539127. Epub 2016 Jan 19.
Formaldehyde (FA) is the leading cause of cellular injury and oxidative damage in testis that is one of the main infertility causes. There has been an increasing evidence of herbal remedies use in male infertility treatment. This assay examines the role of Ficus carica (Fc) leaf extracts in sperm parameters and testis of mice intoxicated with FA. Twenty-five adult male mice were randomly divided into control; sham; FA-treated (10 mg/kg twice per day); Fc-treated (200 mg/kg); and FA + Fc-treated groups. Cauda epididymal spermatozoa were analyzed for viability, count, and motility. Testes were weighed and gonadosomatic index (GSI) was calculated. Also, histoarchitecture of seminiferous tubules was assessed in the Haematoxylin and Eosin stained paraffin sections. The findings showed that FA significantly decreased GSI and increased percentage of immotile sperm compared with control group. Disorganized and vacuolated seminiferous epithelium, spermatogenic arrest, and lumen filled with immature germ cells were also observed in the testes. However, Fc leaf extracts improved sperm count, nonprogressive motility of spermatozoa, and GSI in FA-treated testes. Moreover, seminiferous tubule with spermatogenic arrest was rarely seen, indicating that Fc has the positive effects on testis and epididymal sperm parameters exposed with FA.
甲醛（FA）是睾丸细胞损伤和氧化损伤的主要原因，而睾丸是主要的不孕原因之一。越来越多的证据表明草药疗法可用于治疗男性不育。本试验研究了无花果（Fc）叶提取物对受FA毒害的小鼠精子参数和睾丸的作用。将25只成年雄性小鼠随机分为对照组、假手术组、FA处理组（10 mg/kg，每天两次）、Fc处理组（200 mg/kg）和FA + Fc处理组。分析附睾尾部精子的活力、数量和运动能力。称量睾丸重量并计算性腺体指数（GSI）。此外，在苏木精和伊红染色的石蜡切片中评估生精小管的组织结构。结果表明，与对照组相比，FA显著降低了GSI，并增加了不活动精子的百分比。在睾丸中还观察到生精上皮紊乱、空泡化、生精停滞以及管腔中充满未成熟生殖细胞。然而，Fc叶提取物改善了FA处理组睾丸中的精子数量、精子的非进行性运动能力和GSI。此外，很少见到生精停滞的生精小管，这表明Fc对暴露于FA的睾丸和附睾精子参数具有积极影响。
