Wu Yan, Gu Yan, Guo Shanyu, Dai Qiancheng, Zhang Wei
Department of Surgery, The Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China; Department of Surgery, Zhoupu Hospital of Pudong District, Shanghai, China.
Department of Surgery, The Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
Biomed Res Int. 2016;2016:7593787. doi: 10.1155/2016/7593787. Epub 2016 Jan 21.
ARID1A is one of the important cancer-related genes and regulates transcription of certain genes by altering chromatin structure. Inactivated mutations and decreased expression of ARID1A gene have been reported in several kinds of cancer. Histone H2B is a major component of chromatin and encoded by HIST1H2BE. The goal of the study is to evaluate expressing status of ARID1A and H2B as well as their correlation on breast cancer. Gene expression profiles of ARID1A and H2B on Oncomine database are analyzed. Tissue microarray of breast cancer was used for examination of ARID1A and H2B expression by immunohistochemistry. As a result, the disagreement of ARID1A expression was found, while HIST1H2BE expression is elevated in 4 out of 5 datasets on Oncomine database. There were 15 cases (20%) of breast cancers that were positive for ARID1A. Fifty-eight out of 75 cases of breast cancer (77.3%) were highly expressed for H2B protein and 17 cases (22.7%) were low expressed for H2B protein. All cases with ARID1A expression are overlapped with H2B high expression. Among 15 cases with ARID1A and H2B coexpression, 13 are invasive ductal carcinoma and 2 are mucinous carcinoma. Our results indicate that ARID1A gene may be involved in carcinogenesis of some subtypes of breast cancer.
ARID1A是重要的癌症相关基因之一，通过改变染色质结构来调控某些基因的转录。在多种癌症中均有报道ARID1A基因的失活突变和表达降低。组蛋白H2B是染色质的主要成分之一，由HIST1H2BE编码。本研究的目的是评估ARID1A和H2B在乳腺癌中的表达状态及其相关性。分析了Oncomine数据库中ARID1A和H2B的基因表达谱。利用乳腺癌组织芯片通过免疫组织化学检测ARID1A和H2B的表达。结果发现，Oncomine数据库中5个数据集中有4个数据集的ARID1A表达存在差异，而HIST1H2BE表达升高。15例（20%）乳腺癌病例的ARID1A呈阳性。75例乳腺癌病例中有58例（77.3%）H2B蛋白高表达，17例（22.7%）H2B蛋白低表达。所有ARID1A表达阳性的病例均与H2B高表达重叠。在15例ARID1A和H2B共表达的病例中，13例为浸润性导管癌，2例为黏液癌。我们的结果表明，ARID1A基因可能参与了某些亚型乳腺癌的致癌过程。
