Yersal Ozlem, Malatyali Erdogan, Ertabaklar Hatice, Oktay Esin, Barutca Sabri, Ertug Sema
Division of Medical Oncology, Department of Internal Medicine, Adnan Menderes University, Aydın, Turkey.
Department of Parasitology, Faculty of Medicine, Adnan Menderes University, Aydın, Turkey.
Parasitol Int. 2016 Dec;65(6 Pt B):792-796. doi: 10.1016/j.parint.2016.02.010. Epub 2016 Feb 21.
The aim of the present study was to examine the frequency and genetic diversity of Blastocystis in cancer patients of a Medical Oncology Department in Aydın, Turkey. Patients' stool samples were examined between January 2013 and February 2014 by both microscopy and culture methods. Culture positive samples were subjected to DNA isolation and Sequence Tagged Site (STS)-PCR analysis. Possible etiological factors and clinical features of Blastocystis infection were also analyzed and compared between Blastocystis infected and non-infected subgroups. Blastocystis was detected in 15 (6.5%) of 232 stool samples by microscopy and in 25 (10.8%) by culture methods. Out of 25 culture positive isolates, the most prevalent subtype was ST3 (59%), followed by ST1 (23%) and ST2 (18%). Blastocystis frequency was higher in the male patients than the females (19% vs. 6.5%, p<0.05) and in the patients living in urban areas than rural (15.3% vs. 6.6%, p<0.05). Interestingly, Blastocystis was more frequent in patients with lung cancer than the other cancer types (χ=18, p<0.05) and also in the patients who had received at least eight chemotherapy cycles than fewer (21.4% vs. 9.9%, p<0.05). The rate of gastrointestinal symptoms was not significantly different between infected and non-infected cases. The pathogenic and clinical impacts of Blastocystis in cancer patients should be further examined, particularly as relates to treatment, microbiota and cancer type.
本研究的目的是检测土耳其艾登市某医学肿瘤科癌症患者中芽囊原虫的感染频率及基因多样性。于2013年1月至2014年2月期间，采用显微镜检查和培养方法对患者的粪便样本进行检测。对培养阳性的样本进行DNA提取及序列标签位点（STS）-PCR分析。同时，分析并比较芽囊原虫感染组和未感染组可能的病因及临床特征。通过显微镜检查，在232份粪便样本中检测到15份（6.5%）芽囊原虫；通过培养方法检测到25份（10.8%）。在25份培养阳性分离株中，最常见的亚型为ST3（59%），其次是ST1（23%）和ST2（18%）。男性患者的芽囊原虫感染率高于女性（19% 对6.5%，p<0.05），居住在城市地区的患者高于农村地区（15.3% 对6.6%，p<0.05）。有趣的是，肺癌患者中的芽囊原虫感染率高于其他癌症类型（χ=18，p<0.05），接受至少8个化疗周期的患者高于接受较少化疗周期的患者（21.4% 对9.9%，p<0.05）。感染组和未感染组的胃肠道症状发生率无显著差异。芽囊原虫对癌症患者的致病性和临床影响有待进一步研究，尤其是在治疗、微生物群和癌症类型方面。
