Molina-Manso Diana, Del-Prado Gema, Gómez-Barrena Enrique, Cordero-Ampuero Jose, Fernandez-Roblas Ricardo, Esteban Jaime
Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain.
Department of Orthopaedic Surgery, IdIPaz, Hospital Universitario La Paz, Madrid, Spain.
J Antibiot (Tokyo). 2016 Sep;69(9):686-8. doi: 10.1038/ja.2016.9. Epub 2016 Feb 24.
An in vitro study aimed to evaluate the effect of N-acetyl cysteine (NAC) or sub-ICs of erythromycin on antimicrobial susceptibility of staphylococcal biofilms was performed. Staphylococcus aureus and Staphylococcus epidermidis strains were isolated from patients with prosthetic joint infections using a previously published sonication procedure. Conventional susceptibility studies were performed using microdilution according to the CLSI procedures. Biofilm susceptibility was performed using the Calgary methodology. The addition of NAC showed no effect with the S. aureus strains, and a strain-dependent effect with the S. epidermidis strains. No effect was detected with erythromycin for almost all tested strains.
进行了一项体外研究，旨在评估N-乙酰半胱氨酸（NAC）或亚抑菌浓度的红霉素对葡萄球菌生物膜抗菌敏感性的影响。使用先前发表的超声处理程序从人工关节感染患者中分离出金黄色葡萄球菌和表皮葡萄球菌菌株。根据CLSI程序，采用微量稀释法进行常规药敏试验。使用卡尔加里方法进行生物膜药敏试验。添加NAC对金黄色葡萄球菌菌株无影响，对表皮葡萄球菌菌株有菌株依赖性影响。几乎所有测试菌株对红霉素均无反应。
