Wan-Mohtar Wan Abd Al Qadr Imad, Young Louise, Abbott Gráinne M, Clements Carol, Harvey Linda M, McNeil Brian
Fermentation Centre, Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), Strathclyde University, Glasgow G4 ORE, UK.
Strathclyde Institute for Drug Research (SIDR), SIPBS, Strathclyde University, Glasgow G4 0RE, UK.
J Microbiol Biotechnol. 2016 Jun 28;26(6):999-1010. doi: 10.4014/jmb.1510.10018.
Ganoderma lucidum BCCM 31549 has a long established role for its therapeutic activities. In this context, much interest has focused on the possible functions of the (1,3)-β-D-glucan (G) produced by these cultures in a stirred-tank bioreactor and extracted from their underutilized mycelium. In the existing study, we report on the systematic production of G, and its sulfated derivative (GS). The aim of this study was to investigate G and its GS from G. lucidum in terms of their antibacterial properties and cytotoxicity spectrum against human prostate cells (PN2TA) and human caucasian histiocytic lymphoma cells (U937). (1)H NMR for both G and GS compounds showed β-glycosidic linkages and structural similarities when compared with two standards (laminarin and fucoidan). The existence of characteristic absorptions at 1,170 and 867 cm(-1) in the FTIR (Fourier Transform Infrared Spectroscopy) for GS demonstrated the successful sulfation of G. Only GS exhibited antimicrobial activity against a varied range of test bacteria of relevance to foodstuffs and human health. Moreover, both G and GS did not show any cytotoxic effects on PN2TA cells, thus helping demonstrate the safety of these polymers. Moreover, GS showed 40% antiproliferation against cancerous U937 cells at the low concentration (60 μg/ ml) applied in this study compared with G (10%). Together, this demonstrates that sulfation clearly improved the solubility and therapeutic activities of G. The water-soluble GS demonstrates the potential multifunctional effects of these materials in foodstuffs.
灵芝BCCM 31549因其治疗活性而长期发挥作用。在此背景下，人们对这些培养物在搅拌罐生物反应器中产生并从未充分利用的菌丝体中提取的（1,3）-β-D-葡聚糖（G）的可能功能产生了浓厚兴趣。在现有研究中，我们报告了G及其硫酸化衍生物（GS）的系统生产。本研究的目的是研究来自灵芝的G及其GS对人类前列腺细胞（PN2TA）和人类白种人组织细胞淋巴瘤细胞（U937）的抗菌特性和细胞毒性谱。G和GS化合物的（1）H NMR显示，与两种标准品（海带多糖和岩藻依聚糖）相比，存在β-糖苷键和结构相似性。GS在傅里叶变换红外光谱（FTIR）中1170和867 cm（-1）处存在特征吸收，证明了G的成功硫酸化。只有GS对一系列与食品和人类健康相关的测试细菌表现出抗菌活性。此外，G和GS对PN2TA细胞均未显示任何细胞毒性作用，从而有助于证明这些聚合物的安全性。此外，在本研究中应用的低浓度（60μg/ml）下，GS对癌性U937细胞的抗增殖率为40%，而G为10%。综上所述，这表明硫酸化明显提高了G的溶解度和治疗活性。水溶性GS证明了这些材料在食品中的潜在多功能作用。
