蘑菇菌丝体硫酸葡聚糖的抗真菌-去黑色素特性及对RAW264.7巨噬细胞的刺激作用
Antifungal-demelanizing properties and RAW264.7 macrophages stimulation of glucan sulfate from the mycelium of the mushroom .
作者信息
Wan-Mohtar Wan Abd Al Qadr Imad, Viegelmann Christina, Klaus Anita, Lim Sarina Abdul Halim
机构信息
1Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 50603 Malaysia.
2Fermentation Centre, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, Scotland, U.K. G4 0RE UK.
出版信息
Food Sci Biotechnol. 2017 Feb 28;26(1):159-165. doi: 10.1007/s10068-017-0021-6. eCollection 2017.
Underutilized mycelium of BCCM 31549 has been a significant source of a glucan sulfate (GS) possessing therapeutic activities. GS have been evaluated for their antifungaldemelanizing properties and nitrite oxide production from stimulated RAW264.7 macrophages. GS exhibited antifungal activity against A60 with a minimum inhibitory concentration of 60 mg/mL and a minimum fungicidal concentration of 100 mg/mL. At 60mg/mL (sublethal) and 30mg/mL (subinhibitory) doses of GS, the mycelium of A. niger A60 was successfully demelanized with a conidiophore head and black pigment reduction. Additionally, GS successfully stimulated RAW264.7 macrophage cells at a concentration of 500 μg/mL to produce 0.45 μM of nitric oxide. The GS-stimulated RAW264.7 macrophages were morphologically similar to those treated with lipopolysaccharide. The results highlight a novel bifunctional property of mycelial GS from G. lucidum.
未充分利用的BCCM 31549菌丝体一直是具有治疗活性的硫酸葡聚糖（GS）的重要来源。已对GS的抗真菌、去黑色素特性以及刺激RAW264.7巨噬细胞产生一氧化氮的能力进行了评估。GS对A60表现出抗真菌活性，最低抑菌浓度为60 mg/mL，最低杀菌浓度为100 mg/mL。在60mg/mL（亚致死剂量）和30mg/mL（亚抑制剂量）的GS剂量下，黑曲霉A60的菌丝体成功实现了去黑色素，分生孢子头和黑色色素减少。此外，GS在浓度为500 μg/mL时成功刺激RAW264.7巨噬细胞产生0.45 μM的一氧化氮。GS刺激的RAW264.7巨噬细胞在形态上与用脂多糖处理的细胞相似。结果突出了来自灵芝的菌丝体GS的一种新型双功能特性。
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