Wang Liqiang, Ma Ruijue, Du Gaiping, Guo Huiling, Huang Yifei
Department of Ophthalmology, Chinese PLA General Hospital, Beijing, 100853, China.
J Biomed Mater Res B Appl Biomater. 2015 Jan;103(1):204-11. doi: 10.1002/jbm.b.33192. Epub 2014 May 13.
Silk proteins represent a unique choice in the selection of biomaterials that can be used for corneal tissue engineering and regenerative medical applications. We implanted helicoidal multilamellar arginine-glycine-aspartic acid-functionalized silk biomaterials into the corneal stroma of rabbits, and evaluated its biocompatibility. The corneal tissue was examined after routine hematoxylin-eosin staining, immunofluorescence for collagen I and III, and fibronectin, and scanning electron microscopy. The silk films maintained their integrity and transparency over the 180-day experimental period without causing immunogenic and neovascular responses or degradation of the rabbit corneal stroma. Collagen I increased, whereas Collagen III and fibronectin initially increased and then gradually decreased. The extracellular matrix deposited on the surface of the silk films, tightly adhered to the biomaterial. We have shown this kind of silk film graft has suitable biocompatibility with the corneal stroma and is an initial step for clinical trials to evaluate this material as a transplant biomaterial for keratoplasty tissue constructs.
丝蛋白是用于角膜组织工程和再生医学应用的生物材料选择中的独特之选。我们将螺旋状多层精氨酸-甘氨酸-天冬氨酸功能化丝生物材料植入兔角膜基质，并评估其生物相容性。常规苏木精-伊红染色、I型和III型胶原以及纤连蛋白的免疫荧光检查以及扫描电子显微镜检查后对角膜组织进行了检查。丝膜在180天的实验期内保持其完整性和透明度，未引起兔角膜基质的免疫原性和新生血管反应或降解。I型胶原增加，而III型胶原和纤连蛋白最初增加，然后逐渐减少。沉积在丝膜表面的细胞外基质紧密粘附于生物材料。我们已经表明这种丝膜移植物与角膜基质具有合适的生物相容性，并且是将该材料评估为角膜移植组织构建体的移植生物材料的临床试验的第一步。
