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重组七鳃鳗CRBGP对人脐静脉内皮细胞（HUVECs）管腔形成及鸡胚绒毛尿囊膜（CAM）模型中新血管生成的抑制作用数据。

Data for the inhibition effects of recombinant lamprey CRBGP on the tube formation of HUVECs and new blood vessel generation in CAM models.

作者信息

Jiang Qi, Liu Yu, Gou Meng, Han Jianmei, Wang Jihong, Li Qingwei, Xiao Rong

机构信息

School of Life Sciences, Liaoning Normal University, Dalian 116081, PR China; Lamprey Research Center, Liaoning Normal University, Dalian 116081, PR China.

出版信息

Data Brief. 2016 Jan 13;6:661-7. doi: 10.1016/j.dib.2016.01.004. eCollection 2016 Mar.

DOI:10.1016/j.dib.2016.01.004
PMID:26909383
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4735471/
Abstract

In the present data article, lamprey cysteine-rich buccal gland protein (CRBGP) which belongs to cysteine-rich secretory proteins (CRISPs) family was recombinant and expressed in Rosetta blue cells. After identification, the recombinant protein was purified through affinity chromatograph. The inhibition effects of recombinant lamprey CRBGP (rL-CRBGP) on tube formation of human umbilical vein endothelial cells (HUVECs) and new blood vessel generation in chick chorioallantoic membrane (CAM) models were analyzed. This paper contains data related to research concurrently published in "Anti-angiogenic activities of CRBGP from buccal glands of lampreys (Lampetra japonica)" [1].

摘要

在本数据文章中，属于富含半胱氨酸分泌蛋白（CRISPs）家族的七鳃鳗富含半胱氨酸的颊腺蛋白（CRBGP）在Rosetta blue细胞中进行了重组表达。鉴定后，通过亲和色谱法对重组蛋白进行了纯化。分析了重组七鳃鳗CRBGP（rL-CRBGP）对人脐静脉内皮细胞（HUVECs）管形成以及鸡胚绒毛尿囊膜（CAM）模型中新生血管生成的抑制作用。本文包含与同时发表在《日本七鳃鳗颊腺CRBGP的抗血管生成活性》[1]中的研究相关的数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2da/4735471/ba693adc13fb/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2da/4735471/a60eecfd21f0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2da/4735471/7425f7f2a2fa/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2da/4735471/dcf34707e625/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2da/4735471/589037b01a56/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2da/4735471/ba693adc13fb/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2da/4735471/a60eecfd21f0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2da/4735471/7425f7f2a2fa/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2da/4735471/dcf34707e625/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2da/4735471/589037b01a56/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2da/4735471/ba693adc13fb/gr5.jpg

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本文引用的文献

1
Anti-angiogenic activities of CRBGP from buccal glands of lampreys (Lampetra japonica).日本七鳃鳗颊腺中CRBGP的抗血管生成活性
Biochimie. 2016 Apr;123:7-19. doi: 10.1016/j.biochi.2015.11.020. Epub 2015 Nov 23.
2
Anti-angiogenic activities of snake venom CRISP isolated from Echis carinatus sochureki.从锯鳞蝰蛇（Echis carinatus sochureki）中分离出的蛇毒富含半胱氨酸的分泌蛋白（CRISP）的抗血管生成活性。
Biochim Biophys Acta. 2015 Jun;1850(6):1169-79. doi: 10.1016/j.bbagen.2015.02.002. Epub 2015 Feb 7.
3
Novel cysteine-rich secretory protein in the buccal gland secretion of the parasitic lamprey, Lethenteron japonicum.
日本七鳃鳗（Lethenteron japonicum）口腔腺分泌物中的新型富含半胱氨酸分泌蛋白。
Biochem Biophys Res Commun. 2007 Jun 22;358(1):35-40. doi: 10.1016/j.bbrc.2007.04.065. Epub 2007 Apr 19.
4
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.CLUSTAL_X 窗口界面：借助质量分析工具的多序列比对灵活策略。
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82. doi: 10.1093/nar/25.24.4876.