Ulus Gönül, Koparal A Tansu, Baysal Kemal, Yetik Anacak Günay, Karabay Yavaşoğlu N Ülkü
Department of Biology, Faculty of Science, Ege University, Izmir, Turkey.
Department of Biology, Faculty of Science, Anadolu University, Eskisehir, Turkey.
Cytotechnology. 2018 Dec;70(6):1537-1550. doi: 10.1007/s10616-018-0247-z. Epub 2018 Aug 19.
Cotton, a staple fiber that grows around the seeds of the cotton plants (Gossypium), is produced throughout the world, and its by products, such as cotton fibers, cotton-seed oil, and cottonseed proteins, have a variety of applications. Cotton-seed contains gossypol, a natural phenol compound. (±)-Gossypol is a yellowish polyphenol that is derived from different parts of the cotton plant and contains potent anticancer properties. Tumor growth and metastasis are mainly related to angiogenesis; therefore, anti-angiogenic therapy targets the new blood vessels that provide oxygen and nutrients to actively proliferating tumor cells. The aim of the present study was to evaluate the anti-angiogenic potential of (±)-gossypol in vitro. (±)-Gossypol has anti-proliferative effects on cancer cell lines; however, its anti-angiogenic effects on normal cells have not been studied. Anti-proliferative activities of gossypol assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, anti-angiogenic activities using tube formation assay, and cell migration inhibition capability using a wound-healing assay on human umbilical vein endothelial cells (HUVECs) were revealed. (±)-Gossypol displayed the following potent anti-angiogenic activities in vitro: it inhibited the cell viability of HUVECs, it inhibited the migration of HUVECs, and disrupted endothelial tube formation in a dose-dependent manner. In addition, the anti-angiogenic effects of (±)-gossypol were investigated in ovo in a model using a chick chorioallantoic membrane (CAM). Decreases in capillary density were assessed and scored. (±)-Gossypol showed dose-dependent anti-angiogenic effects on CAM. These findings suggest that (±)-gossypol can be used as a new anti-angiogenic agent.
棉花是一种围绕棉属植物种子生长的主要纤维，在世界各地均有种植，其副产品，如棉纤维、棉籽油和棉籽蛋白，有着多种用途。棉籽含有棉酚，一种天然酚类化合物。（±）-棉酚是一种淡黄色多酚，源自棉花植株的不同部位，具有强大的抗癌特性。肿瘤生长和转移主要与血管生成有关；因此，抗血管生成疗法针对的是为活跃增殖的肿瘤细胞提供氧气和营养的新血管。本研究的目的是评估（±）-棉酚在体外的抗血管生成潜力。（±）-棉酚对癌细胞系具有抗增殖作用；然而，其对正常细胞的抗血管生成作用尚未得到研究。通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）法评估棉酚的抗增殖活性，通过管形成试验评估抗血管生成活性，并通过伤口愈合试验揭示其对人脐静脉内皮细胞（HUVECs）的细胞迁移抑制能力。（±）-棉酚在体外表现出以下强大的抗血管生成活性：它抑制HUVECs的细胞活力，抑制HUVECs的迁移，并以剂量依赖性方式破坏内皮管形成。此外，在使用鸡胚绒毛尿囊膜（CAM）的模型中，在卵内研究了（±）-棉酚的抗血管生成作用。评估并记录毛细血管密度的降低情况。（±）-棉酚对CAM显示出剂量依赖性的抗血管生成作用。这些发现表明（±）-棉酚可作为一种新型抗血管生成剂。
